#1: Journal: J.Gen.Virol. / Year: 1998 Title: A Structural Model of Picornaviral Leader Proteinases Based on Papain and Bleomycin Hydrolase Authors: Skern, T. / Fita, I. / Guarne, A.
History
Deposition
Nov 13, 1999
Deposition site: PDBE / Processing site: PDBE
Revision 1.0
Nov 10, 2000
Provider: repository / Type: Initial release
Revision 1.1
May 8, 2011
Group: Version format compliance
Revision 1.2
Jul 13, 2011
Group: Version format compliance
Revision 1.3
Jul 24, 2019
Group: Data collection / Category: diffrn_source / Item: _diffrn_source.pdbx_synchrotron_site
Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
Sequence details
THE SWISSPROT ENTRY POLG_FMDVO REFERS TO A GENOME POLYPROTEIN, AND THE PROTEASE (EC 3.4.22.-) ...THE SWISSPROT ENTRY POLG_FMDVO REFERS TO A GENOME POLYPROTEIN, AND THE PROTEASE (EC 3.4.22.-) LISTED IS FOR THE RESIDUES 1650 TO 1862. THE PROTEASE STUDIED HERE WAS FOUND IN THE RESIDUES 29 TO 195 THAT ARE LISTED IN SWISSPROT AS A NONSTRUCTURAL PROTEIN P20A.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3 Å3/Da / Density % sol: 58.9 %
Crystal grow
pH: 8.5 Details: 10% PEG 6000, 0.8 M MG CHLORIDE, 0.1 M TRIS/HCL PH=8.5, pH 8.50
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.8469 Å / Relative weight: 1
Reflection
Resolution: 3→34.7 Å / Num. obs: 35080 / % possible obs: 92.5 % / Redundancy: 8.4 % / Biso Wilson estimate: 63.7 Å2 / Rsym value: 0.01 / Net I/σ(I): 9.5
Reflection shell
Resolution: 3→3.05 Å / Mean I/σ(I) obs: 2.9 / Rsym value: 0.263 / % possible all: 74.9
Reflection
*PLUS
Rmerge(I) obs: 0.103
Reflection shell
*PLUS
% possible obs: 75 % / Rmerge(I) obs: 0.263
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Processing
Software
Name
Version
Classification
CNS
0.9
refinement
DENZO
datareduction
SCALEPACK
datascaling
AMoRE
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3→19.99 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 2758596.51 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 Details: NATIVE PATTERSON MAP PRESENT A PSEUDO-ORIGEN PEAK AT POSITION (0.5,0.5,0.225). THIS PEAK CORRESPONDS A PURE TRANSLATION THAT RELATES FOUR MOLECULES OF THE A.S.U. WITH THE OTHER FOUR
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