Mass: 45353.691 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Tissue: BLOOD PLASMA / Description: THE MUTATION IS A NATURALLY OCCURRING VARIANT / Plasmid: PZM-S / Cellular location (production host): CYTOPLASM / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): N4830-1 / References: UniProt: P01011
Compound details
RESIDUE 55 HAS BEEN MUTATED FROM LEU TO PRO. THIS MUTANT IS A NATURALLY OCCURRING VARIANT WHICH HAS ...RESIDUE 55 HAS BEEN MUTATED FROM LEU TO PRO. THIS MUTANT IS A NATURALLY OCCURRING VARIANT WHICH HAS BEEN ASSOCIATED WITH PLASMA DEFICIENCY AND CHRONIC OBSTRUCTIVE PULMONARY DISEASE.
Sequence details
MET 0, PART OF EXPRESSION VECTOR ALA 1, PART OF EXPRESSION VECTOR SER 2, PART OF EXPRESSION VECTOR ...MET 0, PART OF EXPRESSION VECTOR ALA 1, PART OF EXPRESSION VECTOR SER 2, PART OF EXPRESSION VECTOR RESIDUES THR 226A AND LEU 278A ARE NUMBERED WITH AN INSERT CODE TO MATCH THE RESIDUE NUMBERING IN PDB ENTRY 1AS4
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.31 Å3/Da / Density % sol: 47 %
Crystal grow
Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 1 MICROLITER OF 10MG/ML PROTEIN IN 50MM TRIS, 50MM KCL, PH 7.4 WAS MIXED WITH 2 MICROLITER OF PRECIPITANT AND EQUILIBRATED AS A HANGING DROP OVER 1ML OF PRECIPITANT (20% [W/V] PEG 4000, 0.2M ...Details: 1 MICROLITER OF 10MG/ML PROTEIN IN 50MM TRIS, 50MM KCL, PH 7.4 WAS MIXED WITH 2 MICROLITER OF PRECIPITANT AND EQUILIBRATED AS A HANGING DROP OVER 1ML OF PRECIPITANT (20% [W/V] PEG 4000, 0.2M AMMONIUM SULPHATE, 0.1M NAOAC, PH 4.5), AT 18 DEGREES C
Resolution: 2.27→40.95 Å / Data cutoff high absF: 1393995.53 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: MLF Details: RESIDUES 86-89, 104-110, 122-125, AND 212-214 HAD VERY WEAK DENSITY. THEY HAVE BEEN LEFT IN THE CONFORMATION OF THE MOLECULAR REPLACEMENT SEARCH MODEL (1AS4) WHICH WAS DETERMINED AT HIGHER ...Details: RESIDUES 86-89, 104-110, 122-125, AND 212-214 HAD VERY WEAK DENSITY. THEY HAVE BEEN LEFT IN THE CONFORMATION OF THE MOLECULAR REPLACEMENT SEARCH MODEL (1AS4) WHICH WAS DETERMINED AT HIGHER RESOLUTION. HOWEVER, STEREOCHEMICAL PROBLEMS DO OCCUR IN THESE REGIONS. THE DENSITY FOR THE SIDE CHAIN ATOMS OF RESIDUES: LYS 162, ILE 173, SER 348, THR 351, LEU 358 AND SER 359 WAS ABSENT. THEREFORE NO ATOMS BEYOND CB HAVE BEEN INCLUDED IN THE MODEL. ALA 398 WAS NOT VISIBLE IN THE DENSITY
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