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Open data
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Basic information
Entry | Database: PDB / ID: 4caa | |||||||||
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Title | CLEAVED ANTICHYMOTRYPSIN T345R | |||||||||
![]() | (ANTICHYMOTRYPSIN) x 2 | |||||||||
![]() | SERPIN / SERINE PROTEASE INHIBITOR / ANTICHYMOTRYPSIN | |||||||||
Function / homology | ![]() maintenance of gastrointestinal epithelium / regulation of lipid metabolic process / platelet alpha granule lumen / acute-phase response / serine-type endopeptidase inhibitor activity / azurophil granule lumen / Platelet degranulation / secretory granule lumen / collagen-containing extracellular matrix / blood microparticle ...maintenance of gastrointestinal epithelium / regulation of lipid metabolic process / platelet alpha granule lumen / acute-phase response / serine-type endopeptidase inhibitor activity / azurophil granule lumen / Platelet degranulation / secretory granule lumen / collagen-containing extracellular matrix / blood microparticle / inflammatory response / Neutrophil degranulation / DNA binding / extracellular space / extracellular exosome / extracellular region / nucleus Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Lukacs, C.M. / Christianson, D.W. | |||||||||
![]() | ![]() Title: Engineering an anion-binding cavity in antichymotrypsin modulates the "spring-loaded" serpin-protease interaction. Authors: Lukacs, C.M. / Rubin, H. / Christianson, D.W. #1: ![]() Title: Burial of an Ion Pair in the Hydrophobic Core of Cleaved Ala-349->Arg Antichymotrypsin Compromises But Does not Obliterate Serpin Function Authors: Lukacs, C.M. / Rubin, H. / Christianson, D.W. | |||||||||
History |
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Remark 700 | SHEET RESIDUE A 345 IS MUTATION OF THR->ARG. THERE IS AN UNUSUAL TWIST TO THE BETA SHEET IN THIS ...SHEET RESIDUE A 345 IS MUTATION OF THR->ARG. THERE IS AN UNUSUAL TWIST TO THE BETA SHEET IN THIS AREA TO KEEP THE ARG SOLVENT EXPOSED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 84.5 KB | Display | ![]() |
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PDB format | ![]() | 63.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 369.7 KB | Display | ![]() |
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Full document | ![]() | 378.1 KB | Display | |
Data in XML | ![]() | 9.4 KB | Display | |
Data in CIF | ![]() | 13.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1as4C ![]() 3caaC ![]() 1ct3 S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38582.125 Da / Num. of mol.: 1 / Mutation: T345R Source method: isolated from a genetically manipulated source Details: CLEAVED ANTICHYMOTRYPSIN / Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 4359.141 Da / Num. of mol.: 1 / Mutation: T345R Source method: isolated from a genetically manipulated source Details: CLEAVED ANTICHYMOTRYPSIN / Source: (gene. exp.) ![]() ![]() ![]() |
Compound details | A: N-TERMINUS TO CLEAVAGE SITE (RESIDUES 20 - 358) B: CLEAVAGE SITE TO C-TERMINUS (RESIDUES 359 - 393) |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 59 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5.6 Details: 14% PEG 8000 0.2 M MAGNESIUM ACETATE 0.1 M SODIUM CITRATE PH 5.6 | ||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Lukacs, C.M., (1996) Nat.Struct.Biol., 3, 888. | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC / Detector: CCD / Date: Sep 1, 1996 |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→20 Å / Num. obs: 37763 / % possible obs: 95.9 % / Redundancy: 3.35 % / Rsym value: 0.085 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 3.3 % / Rsym value: 0.426 / % possible all: 97.1 |
Reflection | *PLUS Num. obs: 11269 / Num. measured all: 37763 / Rmerge(I) obs: 0.085 |
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Processing
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Refinement | Method to determine structure: DIFFERENCE FOURIER Starting model: PDB ENTRY 1CT3 ![]() 1ct3 Resolution: 2.9→8 Å / Rfactor Rfree error: 0.013 / Data cutoff high absF: 100000 / Data cutoff low absF: 0.1 / Cross valid method: THROUGHOUT / σ(F): 2 Details: GLN A 105 - ASP A 108 ARE IN VERY POOR ELECTRON DENSITY AND SHOULD BE TREATED AS SUCH. THEY HAVE BEEN REFINED WITH OCCUPANCIES OF 0.0. DATA WAS INDEXED WITH B>C IN ORDER TO USE DIFFERENCE ...Details: GLN A 105 - ASP A 108 ARE IN VERY POOR ELECTRON DENSITY AND SHOULD BE TREATED AS SUCH. THEY HAVE BEEN REFINED WITH OCCUPANCIES OF 0.0. DATA WAS INDEXED WITH B>C IN ORDER TO USE DIFFERENCE FOURIER TECHNIQUES FROM ENTRY 1CT3. THE DIFFERENCE IN UNIT CELLS IS PRESUMABLY DUE TO CRYOGENIC TECHNIQUE USED IN THIS DATA SET.
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Displacement parameters | Biso mean: 35.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→3.03 Å / Rfactor Rfree error: 0.067 / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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