[English] 日本語
![](img/lk-miru.gif)
- PDB-1pz0: Structure of NADPH-dependent family 11 aldo-keto reductase AKR11A... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1pz0 | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of NADPH-dependent family 11 aldo-keto reductase AKR11A(holo) | ||||||
![]() | IolS protein | ||||||
![]() | OXIDOREDUCTASE / beta-alpha barrel / aldo-keto reductase / TIM barrel | ||||||
Function / homology | ![]() Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / oxidoreductase activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ehrensberger, A.H. / Wilson, D.K. | ||||||
![]() | ![]() Title: Structural and Catalytic Diversity in the Two Family 11 Aldo-keto Reductases Authors: Ehrensberger, A.H. / Wilson, D.K. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 81.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 60.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 727 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 734.7 KB | Display | |
Data in XML | ![]() | 16.9 KB | Display | |
Data in CIF | ![]() | 23.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Details | One monomer forms the biological unit. |
-
Components
#1: Protein | Mass: 35511.695 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-NA / |
#3: Chemical | ChemComp-NAP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.5 % | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 10.37 Details: PEG 3000, NADP+, pH 10.37, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 10.4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 26, 2003 / Details: Curved crystal |
Radiation | Monochromator: Curved crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95369 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→100 Å / Num. all: 14791 / Num. obs: 13331 / % possible obs: 90.13 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.093 / Net I/σ(I): 17 |
Reflection shell | Resolution: 2.35→2.43 Å / Rmerge(I) obs: 0.218 / Mean I/σ(I) obs: 3.3 / % possible all: 96.1 |
Reflection | *PLUS Num. obs: 14813 / % possible obs: 94.3 % / Num. measured all: 45409 |
Reflection shell | *PLUS % possible obs: 96.1 % |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: AKR11A(apo) Resolution: 2.35→20 Å / Isotropic thermal model: ISOTROPIC / σ(F): 0 / Stereochemistry target values: Engh & Huber
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.35→20 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
LS refinement shell | Resolution: 2.35→2.43 Å / % reflection obs: 96.1 % | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |