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Open data
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Basic information
Entry | Database: PDB / ID: 1phb | ||||||
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Title | INHIBITOR-INDUCED CONFORMATIONAL CHANGE IN CYTOCHROME P450-CAM | ||||||
![]() | CYTOCHROME P450-CAM | ||||||
![]() | OXIDOREDUCTASE(OXYGENASE) | ||||||
Function / homology | ![]() camphor 5-monooxygenase / camphor 5-monooxygenase activity / (+)-camphor catabolic process / cholest-4-en-3-one 26-monooxygenase activity / steroid hydroxylase activity / cholesterol catabolic process / iron ion binding / heme binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Poulos, T.L. | ||||||
![]() | ![]() Title: Inhibitor-induced conformational change in cytochrome P-450CAM. Authors: Raag, R. / Li, H. / Jones, B.C. / Poulos, T.L. #1: ![]() Title: Crystal Structures of Metyrapone-and Phenylimidazole-Inhibited Complexes of Cytochrome P450-Cam Authors: Poulos, T.L. / Howard, A.J. #2: ![]() Title: Crystal Structure of Substrate-Free Pseudomonas Putida Cytochrome P450 Authors: Poulos, T.L. / Finzel, B.C. / Howard, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 99.1 KB | Display | ![]() |
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PDB format | ![]() | 75.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 532.4 KB | Display | ![]() |
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Full document | ![]() | 548.6 KB | Display | |
Data in XML | ![]() | 12.4 KB | Display | |
Data in CIF | ![]() | 19.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE 89 IS A CIS PROLINE. / 2: RESIDUE 100 IS A CIS PROLINE. / 3: RESIDUE 106 IS A CIS PROLINE. / 4: SEE REMARK 8. |
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Components
#1: Protein | Mass: 46588.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Chemical | ChemComp-HEM / |
#3: Chemical | ChemComp-PFZ / |
#4: Water | ChemComp-HOH / |
Nonpolymer details | SOLVENT MOLECULE 515 IS VERY LIKELY A CATION. THE AUTHORS BASED THIS CONCLUSION ON THE OCTAHEDRAL ...SOLVENT MOLECULE 515 IS VERY LIKELY A CATION. THE AUTHORS BASED THIS CONCLUSION |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.16 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 12-15 ℃ / pH: 7 / Method: unknown / Details: Poulos, T.L., (1982) J. Biol. Chem., 257, 10427. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.06 Å / % possible obs: 0.067 % / Num. measured all: 75552 |
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Processing
Software | Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.19 / Highest resolution: 1.6 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.6 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 10 Å / Num. reflection obs: 19152 / Rfactor Rwork: 0.196 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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