+Open data
-Basic information
Entry | Database: PDB / ID: 2h7q | ||||||
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Title | Cytochrome P450cam complexed with imidazole | ||||||
Components | Cytochrome P450-cam | ||||||
Keywords | OXIDOREDUCTASE / Azole drugs / imidazole binding / cytochrome P450 inhibition / protein maleability / active site conformation | ||||||
Function / homology | Function and homology information camphor 5-monooxygenase / camphor 5-monooxygenase activity / (+)-camphor catabolic process / iron ion binding / heme binding / cytoplasm Similarity search - Function | ||||||
Biological species | Pseudomonas putida (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Verras, A. / Alian, A. / Montellano, P.R. | ||||||
Citation | Journal: Protein Eng.Des.Sel. / Year: 2006 Title: Cytochrome P450 active site plasticity: attenuation of imidazole binding in cytochrome P450cam by an L244A mutation. Authors: Verras, A. / Alian, A. / Montellano, P.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2h7q.cif.gz | 108 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2h7q.ent.gz | 80.1 KB | Display | PDB format |
PDBx/mmJSON format | 2h7q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h7/2h7q ftp://data.pdbj.org/pub/pdb/validation_reports/h7/2h7q | HTTPS FTP |
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-Related structure data
Related structure data | 2h7rC 2h7sC 1phcS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 46588.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas putida (bacteria) / Gene: camC, cyp101 / Production host: Escherichia coli (E. coli) / References: UniProt: P00183, camphor 5-monooxygenase |
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#2: Chemical | ChemComp-HEM / |
#3: Chemical | ChemComp-IMD / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.38 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 50mM Potassium phosphate pH 7.4, 250mM KCL, 50mM dithiothreitol, 36-52% ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.6 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Feb 24, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.6 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. all: 73041 / Num. obs: 63319 / % possible obs: 98.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 6.9 % / Rmerge(I) obs: 0.053 / Rsym value: 0.053 / Net I/σ(I): 11.6 |
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 6.5 % / Rmerge(I) obs: 0.432 / Mean I/σ(I) obs: 2.74 / Num. unique all: 63319 / Rsym value: 0.432 / % possible all: 96.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: pDB code 1PHC Resolution: 1.5→35 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.5→35 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.55 Å / Rfactor Rfree error: 0.003
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