[English] 日本語
![](img/lk-miru.gif)
- PDB-1oy8: Structural Basis of Multiple Drug Binding Capacity of the AcrB Mu... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1oy8 | ||||||
---|---|---|---|---|---|---|---|
Title | Structural Basis of Multiple Drug Binding Capacity of the AcrB Multidrug Efflux Pump | ||||||
![]() | Acriflavine resistance protein B | ||||||
![]() | MEMBRANE PROTEIN | ||||||
Function / homology | ![]() xenobiotic detoxification by transmembrane export across the cell outer membrane / efflux pump complex / periplasmic side of plasma membrane / efflux transmembrane transporter activity / xenobiotic transmembrane transporter activity / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yu, E.W. / McDermott, G. / Zgurskaya, H.I. / Nikaido, H. / Koshland Jr., D.E. | ||||||
![]() | ![]() Title: Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Authors: Yu, E.W. / McDermott, G. / Zgurskaya, H.I. / Nikaido, H. / Koshland, D.E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 201.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 160.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 113665.180 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-RHQ / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 4.87 Å3/Da / Density % sol: 74.55 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Temperature: 25 ℃ / pH: 5.6 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Dec 1, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.6→46.62 Å / Num. all: 24586 / Num. obs: 24586 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 |
Reflection | *PLUS Highest resolution: 3.63 Å / Num. obs: 36610 / % possible obs: 100 % / Num. measured all: 260474 / Rmerge(I) obs: 0.101 |
Reflection shell | *PLUS Highest resolution: 3.63 Å / Lowest resolution: 3.78 Å / % possible obs: 99.9 % / Rmerge(I) obs: 0.45 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.63→46.6 Å
| ||||||||||||||||||||
Software | *PLUS Version: 5 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 46.6 Å | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |