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- PDB-1oy6: Structural Basis of the Multiple Binding Capacity of the AcrB Mul... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1oy6 | ||||||
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Title | Structural Basis of the Multiple Binding Capacity of the AcrB Multidrug Efflux Pump | ||||||
![]() | Acriflavine resistance protein B | ||||||
![]() | MEMBRANE PROTEIN | ||||||
Function / homology | ![]() xenobiotic detoxification by transmembrane export across the cell outer membrane / efflux pump complex / periplasmic side of plasma membrane / efflux transmembrane transporter activity / xenobiotic transmembrane transporter activity / outer membrane-bounded periplasmic space / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yu, E.W. / McDermott, G. / Zgurskaya, H.I. / Nikaido, H. / Koshland Jr., D.E. | ||||||
![]() | ![]() Title: Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Authors: Yu, E.W. / McDermott, G. / Zgurskaya, H.I. / Nikaido, H. / Koshland, D.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 198.9 KB | Display | ![]() |
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PDB format | ![]() | 157.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 410.6 KB | Display | ![]() |
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Full document | ![]() | 435.2 KB | Display | |
Data in XML | ![]() | 21.9 KB | Display | |
Data in CIF | ![]() | 32.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 113665.180 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.82 Å3/Da / Density % sol: 74.27 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 25 ℃ / pH: 5.6 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.7→46 Å / Num. all: 34832 / Num. obs: 34832 / % possible obs: 100 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection | *PLUS % possible obs: 100 % / Num. measured all: 204935 / Rmerge(I) obs: 0.086 |
Reflection shell | *PLUS Highest resolution: 3.7 Å / Lowest resolution: 3.83 Å / % possible obs: 100 % / Rmerge(I) obs: 0.444 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 3.68→46 Å
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Software | *PLUS Version: 5 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 3.7 Å / Rfactor Rfree: 0.33 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |