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- PDB-1oy9: Structural Basis of Multiple Drug Binding Capacity of the AcrB Mu... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1oy9 | ||||||
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Title | Structural Basis of Multiple Drug Binding Capacity of the AcrB Multidrug Efflux Pump | ||||||
![]() | Acriflavine resistance protein B | ||||||
![]() | MEMBRANE PROTEIN | ||||||
Function / homology | ![]() xenobiotic detoxification by transmembrane export across the cell outer membrane / efflux pump complex / periplasmic side of plasma membrane / efflux transmembrane transporter activity / xenobiotic transmembrane transporter activity / outer membrane-bounded periplasmic space / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yu, E.W. / McDermott, G. / Zgurskaya, H.I. / Nikaido, H. / Koshland Jr., D.E. | ||||||
![]() | ![]() Title: Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Authors: Yu, E.W. / McDermott, G. / Zgurskaya, H.I. / Nikaido, H. / Koshland, D.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 202.1 KB | Display | ![]() |
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PDB format | ![]() | 159.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 683.2 KB | Display | ![]() |
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Full document | ![]() | 717.3 KB | Display | |
Data in XML | ![]() | 23.4 KB | Display | |
Data in CIF | ![]() | 34.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 113665.180 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ET / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.86 Å3/Da / Density % sol: 74.48 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 25 ℃ / pH: 5.6 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.15→46.58 Å / Num. all: 36512 / Num. obs: 36512 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 |
Reflection | *PLUS Highest resolution: 3.8 Å / Num. obs: 36493 / % possible obs: 99 % / Num. measured all: 280672 / Rmerge(I) obs: 0.101 |
Reflection shell | *PLUS Highest resolution: 3.8 Å / Lowest resolution: 3.94 Å / % possible obs: 100 % / Rmerge(I) obs: 0.442 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 3.8→46.6 Å
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Software | *PLUS Version: 5 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 46.6 Å | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |