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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1oez | ||||||
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タイトル | Zn His46Arg mutant of Human Cu, Zn Superoxide Dismutase | ||||||
![]() | SUPEROXIDE DISMUTASE [CU-ZN] | ||||||
![]() | OXIDOREDUCTASE / HUMAN CU / ZN SUPEROXIDE DISMUTASE | ||||||
機能・相同性 | ![]() action potential initiation / response to antipsychotic drug / neurofilament cytoskeleton organization / response to carbon monoxide / protein phosphatase 2B binding / dense core granule / regulation of organ growth / relaxation of vascular associated smooth muscle / anterograde axonal transport / positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway ...action potential initiation / response to antipsychotic drug / neurofilament cytoskeleton organization / response to carbon monoxide / protein phosphatase 2B binding / dense core granule / regulation of organ growth / relaxation of vascular associated smooth muscle / anterograde axonal transport / positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / response to superoxide / regulation of T cell differentiation in thymus / peripheral nervous system myelin maintenance / retina homeostasis / auditory receptor cell stereocilium organization / hydrogen peroxide biosynthetic process / cellular response to potassium ion / retrograde axonal transport / superoxide anion generation / myeloid cell homeostasis / regulation of GTPase activity / response to copper ion / superoxide metabolic process / muscle cell cellular homeostasis / superoxide dismutase / heart contraction / Detoxification of Reactive Oxygen Species / cellular response to ATP / superoxide dismutase activity / negative regulation of reproductive process / negative regulation of developmental process / transmission of nerve impulse / cellular response to cadmium ion / regulation of multicellular organism growth / ectopic germ cell programmed cell death / response to axon injury / neuronal action potential / ovarian follicle development / positive regulation of superoxide anion generation / regulation of mitochondrial membrane potential / axon cytoplasm / glutathione metabolic process / embryo implantation / dendrite cytoplasm / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / removal of superoxide radicals / reactive oxygen species metabolic process / positive regulation of phagocytosis / placenta development / response to amphetamine / thymus development / positive regulation of cytokine production / determination of adult lifespan / locomotory behavior / response to nutrient levels / response to hydrogen peroxide / sensory perception of sound / mitochondrial intermembrane space / small GTPase binding / regulation of blood pressure / negative regulation of inflammatory response / peroxisome / Platelet degranulation / protein-folding chaperone binding / response to heat / cytoplasmic vesicle / response to ethanol / spermatogenesis / gene expression / negative regulation of neuron apoptotic process / intracellular iron ion homeostasis / lysosome / positive regulation of MAPK cascade / positive regulation of apoptotic process / mitochondrial matrix / response to xenobiotic stimulus / copper ion binding / neuronal cell body / apoptotic process / protein homodimerization activity / protein-containing complex / mitochondrion / extracellular space / extracellular exosome / extracellular region / zinc ion binding / nucleoplasm / identical protein binding / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Strange, R.W. / Antonyuk, S. / Hough, M.A. / Doucette, P. / Rodriguez, J. / Elam, J.S. / Hart, P.J. / Hayward, L.J. / Valentine, J.S. / Hasnain, S.S. | ||||||
![]() | ![]() タイトル: Amyloid-Like Filaments and Water-Filled Nanotubes Formed by Sod1 Mutant Proteins Linked to Familial Als 著者: Elam, J.S. / Taylor, A.B. / Strange, R.W. / Antonyuk, S. / Doucette, P. / Rodriguez, J. / Hasnain, S.S. / Hayward, L.J. / Valentine, J.S. / Yeates, T.O. / Hart, P.J. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 109.3 KB | 表示 | ![]() |
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PDB形式 | ![]() | 85.2 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 467 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 473.4 KB | 表示 | |
XML形式データ | ![]() | 23.3 KB | 表示 | |
CIF形式データ | ![]() | 32.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper:
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要素
#1: タンパク質 | 分子量: 15846.607 Da / 分子数: 4 / 変異: YES / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: 化合物 | ChemComp-ZN / #3: 化合物 | ChemComp-SO4 / #4: 水 | ChemComp-HOH / | 構成要素の詳細 | ENGINEERED | Has protein modification | Y | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.4 Å3/Da / 溶媒含有率: 48 % | ||||||||||||||||||||||||
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結晶化 | pH: 8 / 詳細: 0.2M NA2SO4, 15% PEG 3350, pH 8.00 | ||||||||||||||||||||||||
結晶化 | *PLUS 温度: 25 ℃ / pH: 7 / 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC CCD / 検出器: CCD / 日付: 2002年4月15日 / 詳細: MIRRORS |
放射 | モノクロメーター: SI 111 / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.97 Å / 相対比: 1 |
反射 | 解像度: 2.15→50 Å / Num. obs: 35679 / % possible obs: 99.3 % / 冗長度: 6 % / Rmerge(I) obs: 0.112 / Net I/σ(I): 12 |
反射 シェル | 解像度: 2→2.15 Å / 冗長度: 4 % / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 2 / % possible all: 98.6 |
反射 | *PLUS 最低解像度: 20 Å / Num. obs: 35432 / Num. measured all: 652196 / Rmerge(I) obs: 0.09 |
反射 シェル | *PLUS 最高解像度: 2.15 Å / 最低解像度: 2.21 Å / % possible obs: 98.6 % / Rmerge(I) obs: 0.48 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1HL5 解像度: 2.15→20 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.95 / SU B: 4.548 / SU ML: 0.118 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / ESU R: 0.21 / ESU R Free: 0.174 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS DISORDERED REGIONS IN MONOMERS A, F, D, AND G WERE OMMITED
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 17.85 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2.15→20 Å
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拘束条件 |
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