+Open data
-Basic information
Entry | Database: PDB / ID: 1o8r | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of human proguanylin | ||||||
Components | GUANYLIN | ||||||
Keywords | PROHORMONE / PROGUANYLIN / GUANYLIN / GAP-I / PRECURSOR / PROSEQUENCE | ||||||
Function / homology | Function and homology information guanylate cyclase activator activity / Digestion / hormone activity / extracellular region Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Lauber, T. / Rosch, P. / Marx, U.C. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: Solution Structure of Human Proguanylin: The Role of a Hormone Prosequence Authors: Lauber, T. / Neudecker, P. / Roesch, P. / Marx, U.C. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1o8r.cif.gz | 830.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1o8r.ent.gz | 703.7 KB | Display | PDB format |
PDBx/mmJSON format | 1o8r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1o8r_validation.pdf.gz | 342.5 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1o8r_full_validation.pdf.gz | 627.9 KB | Display | |
Data in XML | 1o8r_validation.xml.gz | 94.7 KB | Display | |
Data in CIF | 1o8r_validation.cif.gz | 122.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/o8/1o8r ftp://data.pdbj.org/pub/pdb/validation_reports/o8/1o8r | HTTPS FTP |
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 10351.790 Da / Num. of mol.: 1 / Fragment: RESIDUES 22-115 Source method: isolated from a genetically manipulated source Details: DISULPHIDE BONDS BETWEEN CYS69 AND CYS82, CYS104 AND CYS112, CYS107 AND CYS115 Source: (gene. exp.) HOMO SAPIENS (human) / Tissue: INTESTINAL MUCOSA AND BLOOD / Description: CLONED FROM CDNA / Cell: ENTERO-ENDOCRINE CELLS / Plasmid: PET32A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): AD494 / Variant (production host): DE3 / References: UniProt: Q02747 |
---|---|
Compound details | ENDOGENOUS ACTIVATOR OF INTESTINAL GUANYLATE CYCLASE. STIMULATES THROUGH THE SAME RECEPTOR BINDING ...ENDOGENOUS |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR details | Text: THE STRUCTURE WAS DETERMINED USING STANDARD 3D NMR EXPERIMENTS ON 15N- AND 13C/15N-LABELED PROGUANYLIN |
-Sample preparation
Crystal grow | *PLUS Method: other / Details: NMR |
---|
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST OVERALL ENERGY, LEAST RESTRAINT VIOLATION Conformers calculated total number: 120 / Conformers submitted total number: 30 |