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Open data
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Basic information
Entry | Database: PDB / ID: 1nps | ||||||
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Title | CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF PROTEIN S | ||||||
![]() | DEVELOPMENT-SPECIFIC PROTEIN S | ||||||
![]() | SIGNALING PROTEIN / CRYSTALLINE LIKE STRUCTURE | ||||||
Function / homology | ![]() sporulation resulting in formation of a cellular spore / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Wenk, M. / Baumgartner, R. / Mayer, E.M. / Huber, R. / Holak, T.A. / Jaenicke, R. | ||||||
![]() | ![]() Title: The domains of protein S from Myxococcus xanthus: structure, stability and interactions. Authors: Wenk, M. / Baumgartner, R. / Holak, T.A. / Huber, R. / Jaenicke, R. / Mayr, E.M. #1: ![]() Title: NMR-Derived Three-Dimensional Solution Structure of Protein S Complexed with Calcium Authors: Bagby, S. / Harvey, T.S. / Eagle, S.G. / Inouye, S. / Ikura, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 30.1 KB | Display | ![]() |
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PDB format | ![]() | 19.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 360.3 KB | Display | ![]() |
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Full document | ![]() | 361.1 KB | Display | |
Data in XML | ![]() | 3.2 KB | Display | |
Data in CIF | ![]() | 4.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 9389.440 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN: MOTIFS 1-2 AND LINKER Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.05 % | ||||||||||||||||||||||||
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Crystal grow | pH: 8 / Details: pH 8.0 | ||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 36 % | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / pH: 7.5 | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: AREA DETECTOR / Date: Jul 1, 1998 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→20 Å / Num. obs: 7166 / % possible obs: 99 % / Observed criterion σ(I): 2 / Redundancy: 2.3 % / Rmerge(I) obs: 0.08 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.8→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 20 Å / σ(F): 1 / % reflection Rfree: 5 % / Rfactor obs: 0.198 / Rfactor Rwork: 0.2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_angle_d / Dev ideal: 0.9 |