+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1mje | ||||||
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タイトル | STRUCTURE OF A BRCA2-DSS1-SSDNA COMPLEX | ||||||
要素 |
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キーワード | GENE REGULATION/ANTITUMOR PROTEIN/DNA / TUMOR SUPPRESSOR (がん抑制遺伝子) / BREAST CANCER SUSCEPTIBILITY / DNA-BINDING / GENE REGULATION-ANTITUMOR PROTEIN-DNA COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 HDR through MMEJ (alt-NHEJ) / Homologous DNA Pairing and Strand Exchange / Presynaptic phase of homologous DNA pairing and strand exchange / Resolution of D-loop Structures through Holliday Junction Intermediates / homologous chromosome orientation in meiotic metaphase I / BRCA2-MAGE-D1 complex / HDR through Homologous Recombination (HRR) / negative regulation of mammary gland epithelial cell proliferation / mitotic recombination-dependent replication fork processing / establishment of protein localization to telomere ...HDR through MMEJ (alt-NHEJ) / Homologous DNA Pairing and Strand Exchange / Presynaptic phase of homologous DNA pairing and strand exchange / Resolution of D-loop Structures through Holliday Junction Intermediates / homologous chromosome orientation in meiotic metaphase I / BRCA2-MAGE-D1 complex / HDR through Homologous Recombination (HRR) / negative regulation of mammary gland epithelial cell proliferation / mitotic recombination-dependent replication fork processing / establishment of protein localization to telomere / Impaired BRCA2 translocation to the nucleus / Impaired BRCA2 binding to SEM1 (DSS1) / nuclear ubiquitin ligase complex / chordate embryonic development / histone H4 acetyltransferase activity / histone H3 acetyltransferase activity / lateral element / telomere maintenance via recombination / regulation of DNA damage checkpoint / mammary gland development / oocyte maturation / proteasome regulatory particle, lid subcomplex / gamma-tubulin binding / DNA repair complex / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Regulation of ornithine decarboxylase (ODC) / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / response to UV-C / Resolution of D-loop Structures through Holliday Junction Intermediates / Cross-presentation of soluble exogenous antigens (endosomes) / inner cell mass cell proliferation / Somitogenesis / DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / hematopoietic stem cell proliferation / Impaired BRCA2 binding to RAD51 / replication fork processing / female gonad development / male meiosis I / Presynaptic phase of homologous DNA pairing and strand exchange / centrosome duplication / 造血 / response to X-ray / chromosome organization / proteasome assembly / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / mRNA export from nucleus / positive regulation of mitotic cell cycle / proteasome complex / Regulation of activated PAK-2p34 by proteasome mediated degradation / regulation of cytokinesis / Autodegradation of Cdh1 by Cdh1:APC/C / 分泌 / APC/C:Cdc20 mediated degradation of Securin / Asymmetric localization of PCP proteins / SCF-beta-TrCP mediated degradation of Emi1 / NIK-->noncanonical NF-kB signaling / stem cell proliferation / Ubiquitin-dependent degradation of Cyclin D / AUF1 (hnRNP D0) binds and destabilizes mRNA / TNFR2 non-canonical NF-kB pathway / Assembly of the pre-replicative complex / Vpu mediated degradation of CD4 / cellular response to ionizing radiation / Degradation of DVL / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Dectin-1 mediated noncanonical NF-kB signaling / nucleotide-excision repair / Hh mutants are degraded by ERAD / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / response to gamma radiation / Degradation of AXIN / Defective CFTR causes cystic fibrosis / Degradation of GLI1 by the proteasome / Hedgehog ligand biogenesis / Activation of NF-kappaB in B cells / Negative regulation of NOTCH4 signaling / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / G2/M Checkpoints / double-strand break repair via homologous recombination / Vif-mediated degradation of APOBEC3G / Autodegradation of the E3 ubiquitin ligase COP1 / Hedgehog 'on' state / brain development / Regulation of RUNX3 expression and activity / Degradation of GLI2 by the proteasome / GLI3 is processed to GLI3R by the proteasome / MAPK6/MAPK4 signaling / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / multicellular organism growth / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / ABC-family proteins mediated transport / Degradation of beta-catenin by the destruction complex / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / HDR through Homologous Recombination (HRR) / CDK-mediated phosphorylation and removal of Cdc6 / CLEC7A (Dectin-1) signaling / SCF(Skp2)-mediated degradation of p27/p21 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) Mus musculus (ハツカネズミ) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 3.5 Å | ||||||
データ登録者 | Yang, H. / Jeffrey, P.D. / Miller, J. / Kinnucan, E. / Sun, Y. / Thoma, N.H. / Zheng, N. / Chen, P.L. / Lee, W.H. / Pavletich, N.P. | ||||||
引用 | ジャーナル: Science / 年: 2002 タイトル: BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure. 著者: Yang, H. / Jeffrey, P.D. / Miller, J. / Kinnucan, E. / Sun, Y. / Thoma, N.H. / Zheng, N. / Chen, P.L. / Lee, W.H. / Pavletich, N.P. | ||||||
履歴 |
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Remark 999 | SEQUENCE RESIDUES 2793-2879 OF THE BRCA2 CHAIN WERE REMOVED PROTEOLYTICALLY. RESIDUES 3087 AND 3089 ...SEQUENCE RESIDUES 2793-2879 OF THE BRCA2 CHAIN WERE REMOVED PROTEOLYTICALLY. RESIDUES 3087 AND 3089 ARE COVALENTLY BOUND. 3088 WAS SKIPPED IN THE NUMBERING. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1mje.cif.gz | 134 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1mje.ent.gz | 103.1 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1mje.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/mj/1mje ftp://data.pdbj.org/pub/pdb/validation_reports/mj/1mje | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
#1: DNA鎖 | 分子量: 1780.199 Da / 分子数: 1 / 由来タイプ: 合成 |
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#2: タンパク質 | 分子量: 8284.611 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: UniProt: P60896 |
#3: タンパク質 | 分子量: 72983.172 Da / 分子数: 1 Fragment: sequence database residues 2378-2792, 2880-3113 (residues 2793-2879 removed) 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: GenBank: 17973451, UniProt: P97929*PLUS |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 5.95 Å3/Da / 溶媒含有率: 79.34 % | ||||||||||||||||||||||||
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結晶化 | 温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.8 詳細: Sodium Acetate, Sodium Citrate, NaCl, DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
溶液の組成 |
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-データ収集
回折 | 平均測定温度: 113 K |
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放射光源 | 由来: シンクロトロン / サイト: CHESS / ビームライン: A1 / 波長: 0.95 Å |
検出器 | タイプ: ADSC QUANTUM 4 / 検出器: CCD / 日付: 2001年10月1日 |
放射 | モノクロメーター: Si(111) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.95 Å / 相対比: 1 |
反射 | 解像度: 3.5→25 Å / Num. all: 24950 / Num. obs: 24950 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
反射 シェル | 解像度: 3.5→3.62 Å / % possible all: 99.2 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 / 解像度: 3.5→15 Å / σ(F): 0 / 立体化学のターゲット値: Engh & Huber
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精密化ステップ | サイクル: LAST / 解像度: 3.5→15 Å
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拘束条件 |
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