+Open data
-Basic information
Entry | Database: PDB / ID: 1m8l | ||||||
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Title | NMR structure of the HIV-1 Regulatory Protein Vpr | ||||||
Components | VPR Protein | ||||||
Keywords | VIRAL PROTEIN / Vpr / CD3CN / HIV-1 | ||||||
Function / homology | Function and homology information symbiont-mediated arrest of host cell cycle during G2/M transition / monoatomic ion transmembrane transport / : / virion component / protein homooligomerization / viral penetration into host nucleus / host extracellular space / symbiont entry into host cell / cell cycle / DNA-templated transcription ...symbiont-mediated arrest of host cell cycle during G2/M transition / monoatomic ion transmembrane transport / : / virion component / protein homooligomerization / viral penetration into host nucleus / host extracellular space / symbiont entry into host cell / cell cycle / DNA-templated transcription / host cell nucleus / regulation of DNA-templated transcription Similarity search - Function | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Morellet, N. / Bouaziz, S. / Lenoir, C. / Roques, B.P. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: NMR Structure of the HIV-1 Regulatory Protein Vpr Authors: Morellet, N. / Bouaziz, S. / Lenoir, C. / Roques, B.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1m8l.cif.gz | 41.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1m8l.ent.gz | 29.2 KB | Display | PDB format |
PDBx/mmJSON format | 1m8l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m8/1m8l ftp://data.pdbj.org/pub/pdb/validation_reports/m8/1m8l | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11411.905 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence is naturally found in human immunodeficiency virus type 1 (HIV-1). References: UniProt: Q73369 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D and 3D homonuclear and heteronuclear techniques |
-Sample preparation
Details | Contents: 1 mM Vpr 70% H2O, 30% CD3CN pH 2.6 / Solvent system: 70% H2O, 30% CD3CN |
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Sample conditions | pH: 2.6 / Pressure: ambient / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total 1115 restraints | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 1 |