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- PDB-1l1j: Crystal structure of the protease domain of an ATP-independent he... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1l1j | ||||||
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Title | Crystal structure of the protease domain of an ATP-independent heat shock protease HtrA | ||||||
![]() | heat shock protease HtrA | ||||||
![]() | HYDROLASE / serine proteinase | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Kim, D.Y. / Kim, D.R. / Ha, S.C. / Lokanath, N.K. / Hwang, H.Y. / Kim, K.K. | ||||||
![]() | ![]() Title: Crystal Structure of the Protease Domain of a Heat-shock Protein HtrA from Thermotoga maritima Authors: Kim, D.Y. / Kim, D.R. / Ha, S.C. / Lokanath, N.K. / Lee, C.J. / Hwang, H.Y. / Kim, K.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96.7 KB | Display | ![]() |
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PDB format | ![]() | 75.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.7 KB | Display | ![]() |
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Full document | ![]() | 459.8 KB | Display | |
Data in XML | ![]() | 21.1 KB | Display | |
Data in CIF | ![]() | 28.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25931.455 Da / Num. of mol.: 2 / Fragment: protease domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q9WZ41, Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.81 Å3/Da / Density % sol: 56.28 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 4.4 Details: phosphate-citrate, Li2SO4, PEG1000, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ | ||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 20 Å / Num. obs: 13843 / % possible obs: 95.1 % / Redundancy: 2.43 % / Rmerge(I) obs: 0.066 |
Reflection shell | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 2.9 Å / % possible obs: 97.2 % / Num. unique obs: 1376 / Rmerge(I) obs: 0.27 |
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Processing
Software | Name: CNS / Version: 1 / Classification: refinement | ||||||||||||
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Refinement | Resolution: 2.8→20 Å
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Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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Refinement | *PLUS Num. reflection all: 13621 / Rfactor Rfree: 0.284 / Rfactor Rwork: 0.222 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS | ||||||||||||
Refine LS restraints | *PLUS
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