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Open data
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Basic information
Entry | Database: PDB / ID: 1ej4 | |||||||||
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Title | COCRYSTAL STRUCTURE OF EIF4E/4E-BP1 PEPTIDE | |||||||||
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![]() | TRANSLATION / eIF4E/4E-BP/7-methyl-GDP | |||||||||
Function / homology | ![]() Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / Deadenylation of mRNA / Translation initiation complex formation / Ribosomal scanning and start codon recognition / ISG15 antiviral mechanism / mTORC1-mediated signalling / L13a-mediated translational silencing of Ceruloplasmin expression ...Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / Deadenylation of mRNA / Translation initiation complex formation / Ribosomal scanning and start codon recognition / ISG15 antiviral mechanism / mTORC1-mediated signalling / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit / Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / eukaryotic initiation factor 4G binding / eukaryotic initiation factor 4E binding / regulation of translation at postsynapse, modulating synaptic transmission / chromatoid body / eukaryotic translation initiation factor 4F complex / mRNA cap binding / RNA 7-methylguanosine cap binding / nuclear export / RISC complex / postsynaptic cytosol / stem cell population maintenance / mTORC1-mediated signalling / negative regulation of neuron differentiation / TOR signaling / behavioral fear response / mRNA export from nucleus / translation repressor activity / negative regulation of translational initiation / translation initiation factor binding / translational initiation / translation initiation factor activity / positive regulation of mitotic cell cycle / cellular response to dexamethasone stimulus / P-body / neuron differentiation / cytoplasmic stress granule / G1/S transition of mitotic cell cycle / regulation of translation / postsynapse / DNA-binding transcription factor binding / nuclear body / negative regulation of translation / nuclear speck / translation / glutamatergic synapse / perinuclear region of cytoplasm / enzyme binding / protein-containing complex / nucleus / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Marcotrigiano, J. / Gingras, A.-C. / Sonenberg, N. / Burley, S.K. | |||||||||
![]() | ![]() Title: Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G. Authors: Marcotrigiano, J. / Gingras, A.C. / Sonenberg, N. / Burley, S.K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.5 KB | Display | ![]() |
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PDB format | ![]() | 39.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 759 KB | Display | ![]() |
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Full document | ![]() | 763.4 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 15.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22145.113 Da / Num. of mol.: 1 / Fragment: RESIDUES 28-217 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1861.240 Da / Num. of mol.: 1 / Fragment: RESIDUES 51-67 / Source method: obtained synthetically Details: THIS PEPTIDE WAS CHEMICALLY SYNTHESIZED. THE SEQUENCE OF THIS PEPTIDE NATURALLY OCCURS IN HUMANS (HOMO SAPIENS) References: GenBank: 4758258, UniProt: Q13541*PLUS |
#3: Chemical | ChemComp-M7G / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.5 % | ||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K | ||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Sep 15, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→30 Å / Num. all: 10817 / % possible obs: 97.4 % / Observed criterion σ(I): 5 / Redundancy: 4 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 24.9 |
Reflection shell | Resolution: 2.25→2.33 Å / Redundancy: 4 % / Rmerge(I) obs: 0.103 / % possible all: 84.8 |
Reflection shell | *PLUS % possible obs: 84.8 % |
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Processing
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Refinement | Resolution: 2.25→25 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.25→25 Å
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Software | *PLUS Name: 'CNS' / Classification: refinement | ||||||||||||
Refine LS restraints | *PLUS
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