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Open data
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Basic information
Entry | Database: PDB / ID: 1jjo | ||||||
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Title | Crystal Structure of Mouse Neuroserpin (Cleaved form) | ||||||
![]() | (NEUROSERPIN) x 3 | ||||||
![]() | SIGNALING PROTEIN / Serpin / Serine protease inhibitor / Neuronal serpin | ||||||
Function / homology | ![]() cytoplasmic vesicle lumen / negative regulation of endopeptidase activity / regulation of cell adhesion / serine-type endopeptidase inhibitor activity / positive regulation of neuron projection development / perikaryon / secretory granule lumen / neuronal cell body / extracellular space Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Briand, C. / Kozlov, S.V. / Sonderegger, P. / Gruetter, M.G. | ||||||
![]() | ![]() Title: Crystal structure of neuroserpin: a neuronal serpin involved in a conformational disease. Authors: Briand, C. / Kozlov, S.V. / Sonderegger, P. / Grutter, M.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 134 KB | Display | ![]() |
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PDB format | ![]() | 106.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 444.9 KB | Display | ![]() |
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Full document | ![]() | 493.4 KB | Display | |
Data in XML | ![]() | 30.5 KB | Display | |
Data in CIF | ![]() | 37.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein/peptide | Mass: 4416.040 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 29630.404 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Protein/peptide | Mass: 3969.749 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.63 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.1 Details: 25% PEG8000, 0.2M H2PO4(NH4), 0.1M Tris-HCl, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 6, 1999 |
Radiation | Monochromator: mirror, Prophysics XRM-216 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3.06→16.86 Å / Num. all: 11124 / Num. obs: 9419 / % possible obs: 79.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 / Redundancy: 2.57 % / Rsym value: 0.155 / Net I/σ(I): 7 |
Reflection shell | Resolution: 3.06→3.1 Å / Redundancy: 2.3 % / Mean I/σ(I) obs: 2 / Num. unique all: 417 / Rsym value: 0.421 / % possible all: 94.6 |
Reflection | *PLUS Lowest resolution: 17 Å / Rmerge(I) obs: 0.155 |
Reflection shell | *PLUS % possible obs: 74.6 % / Num. unique obs: 7975 / Rmerge(I) obs: 0.422 / Mean I/σ(I) obs: 2 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: homology model based on all available cleaved serpin structures Resolution: 3.06→16.89 Å / Isotropic thermal model: anisotropic / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: Bulk solvent correction, strict NCS
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Refinement step | Cycle: LAST / Resolution: 3.06→16.89 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 4.3 % / Rfactor obs: 0.232 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |