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Open data
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Basic information
Entry | Database: PDB / ID: 1jhc | ||||||
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Title | LEXA S119A C-TERMINAL TRYPTIC FRAGMENT | ||||||
![]() | LEXA REPRESSOR | ||||||
![]() | HYDROLASE / LexA SOS repressor | ||||||
Function / homology | ![]() repressor LexA / DNA-binding transcription repressor activity / SOS response / protein-DNA complex / DNA replication / transcription cis-regulatory region binding / serine-type endopeptidase activity / DNA repair / negative regulation of DNA-templated transcription / DNA-templated transcription ...repressor LexA / DNA-binding transcription repressor activity / SOS response / protein-DNA complex / DNA replication / transcription cis-regulatory region binding / serine-type endopeptidase activity / DNA repair / negative regulation of DNA-templated transcription / DNA-templated transcription / DNA damage response / proteolysis / DNA binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Luo, Y. / Pfuetzner, R.A. / Mosimann, S. / Little, J.W. / Strynadka, N.C.J. | ||||||
![]() | ![]() Title: Crystal structure of LexA: a conformational switch for regulation of self-cleavage. Authors: Luo, Y. / Pfuetzner, R.A. / Mosimann, S. / Paetzel, M. / Frey, E.A. / Cherney, M. / Kim, B. / Little, J.W. / Strynadka, N.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 37.8 KB | Display | ![]() |
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PDB format | ![]() | 25.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1jheC ![]() 1jhfC ![]() 1jhhC ![]() 1umuS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | Dimer generated by crystallographic symmetry |
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Components
#1: Protein | Mass: 15178.403 Da / Num. of mol.: 1 / Fragment: C-Terminus, Residues 68-202 / Mutation: S119A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.8 % | |||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.8M potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||
Crystal grow | *PLUS pH: 8.5 | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Oct 20, 2000 / Details: Mirrors |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. all: 10911 / Num. obs: 10911 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9.1 % / Rmerge(I) obs: 0.072 / Rsym value: 0.072 / Net I/σ(I): 32.5 |
Reflection shell | Resolution: 2→2.04 Å / Redundancy: 7.5 % / Rmerge(I) obs: 0.341 / Mean I/σ(I) obs: 6.8 / Num. unique all: 520 / Rsym value: 0.341 / % possible all: 100 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. obs: 9882 / Num. measured all: 90223 |
Reflection shell | *PLUS % possible obs: 100 % / Redundancy: 8.5 % / Num. unique obs: 520 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1UMU Resolution: 2→15 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→15 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 15 Å / σ(F): 0 / Rfactor obs: 0.23 / Rfactor Rwork: 0.23 / % reflection Rfree: 10 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |