+Open data
-Basic information
Entry | Database: PDB / ID: 1ioa | |||||||||
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Title | ARCELIN-5, A LECTIN-LIKE DEFENSE PROTEIN FROM PHASEOLUS VULGARIS | |||||||||
Components | ARCELIN-5A | |||||||||
Keywords | LECTIN / LECTIN-LIKE PROTEINS / PLANT DEFENSE PROTEINS | |||||||||
Function / homology | Function and homology information nutrient reservoir activity / defense response / toxin activity / carbohydrate binding Similarity search - Function | |||||||||
Biological species | Phaseolus vulgaris (French bean) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.7 Å | |||||||||
Authors | Hamelryck, T. / Loris, R. | |||||||||
Citation | Journal: J.Biol.Chem. / Year: 1996 Title: Crystal structure of arcelin-5, a lectin-like defense protein from Phaseolus vulgaris Authors: Hamelryck, T. / Poortmans, F. / Goossens, A. / Angenon, G. / Van Montagu, M. / Wyns, L. / Loris, R. #1: Journal: J.Biol.Chem. / Year: 1996 Title: The Crystallographic Structure of Phytohemagglutinin-L Authors: Hamelryck, T.W. / Dao-Thi, M.H. / Poortmans, F. / Chrispeels, M.J. / Wyns, L. / Loris, R. #2: Journal: Plant Cell / Year: 1991 Title: Lectins, Lectin Genes, and Their Role in Plant Defense Authors: Chrispeels, M.J. / Raikhel, N.V. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ioa.cif.gz | 97 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ioa.ent.gz | 77.7 KB | Display | PDB format |
PDBx/mmJSON format | 1ioa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/io/1ioa ftp://data.pdbj.org/pub/pdb/validation_reports/io/1ioa | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.984554, 0.011087, -0.174727), Vector: |
-Components
#1: Protein | Mass: 26961.941 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Phaseolus vulgaris (French bean) / Organ: SEED / Strain: G02771 / References: PIR: S51359, UniProt: Q42460*PLUS #2: Polysaccharide | Source method: isolated from a genetically manipulated source |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.85 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 4.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER / Date: Feb 22, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 16994 / % possible obs: 97.3 % / Observed criterion σ(I): 0 / Redundancy: 2.9 % / Rmerge(I) obs: 0.085 |
-Processing
Software |
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Refinement | Resolution: 2.7→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 16.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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