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- PDB-1i8f: THE CRYSTAL STRUCTURE OF A HEPTAMERIC ARCHAEAL SM PROTEIN: IMPLIC... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1i8f | ||||||
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Title | THE CRYSTAL STRUCTURE OF A HEPTAMERIC ARCHAEAL SM PROTEIN: IMPLICATIONS FOR THE EUKARYOTIC SNRNP CORE | ||||||
![]() | PUTATIVE SNRNP SM-LIKE PROTEIN | ||||||
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Function / homology | ![]() pICln-Sm protein complex / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mura, C. / Cascio, D. / Sawaya, M.R. / Eisenberg, D. | ||||||
![]() | ![]() Title: The crystal structure of a heptameric archaeal Sm protein: Implications for the eukaryotic snRNP core. Authors: Mura, C. / Cascio, D. / Sawaya, M.R. / Eisenberg, D.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 107.8 KB | Display | ![]() |
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PDB format | ![]() | 85 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The contents of one asymmetric unit (i.e. a heptamer) most likely correspond to the biologically relevant species for this organism. |
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Components
#1: Protein | Mass: 8937.272 Da / Num. of mol.: 7 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-GOL / ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.34 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.3 Details: PEG-4000, acetate, glycerol, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystal grow | *PLUS Method: unknown |
-Data collection
Diffraction | Mean temperature: 105 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 5, 2000 Details: collimating mirror optics, double-slit monochromator |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.71→100 Å / Num. all: 62547 / Num. obs: 62547 / % possible obs: 98.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 6.48 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 44.4 |
Reflection shell | Resolution: 1.71→1.77 Å / Rmerge(I) obs: 0.723 / % possible all: 97.2 |
Reflection | *PLUS Rmerge(I) obs: 0.04 |
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Processing
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Refinement | Method to determine structure![]() ![]() Details: Each of the seven Sm monomers per a.u. were refined independently in CNS since imposition of restraints or constraints hindered the refinement.
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Displacement parameters | Biso mean: 38.5 Å2 | |||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.75→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor obs: 0.235 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 38.5 Å2 | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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