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- PDB-1d3b: CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP D... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1d3b | ||||||
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Title | CRYSTAL STRUCTURE OF THE D3B SUBCOMPLEX OF THE HUMAN CORE SNRNP DOMAIN AT 2.0A RESOLUTION | ||||||
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![]() | RNA BINDING PROTEIN / SNRNP / SPLICING / SM / CORE SNRNP DOMAIN / SYSTEMIC LUPUS ERYTHEMATOSUS / SLE | ||||||
Function / homology | ![]() U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / histone pre-mRNA 3'end processing complex / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / protein methylation / U12-type spliceosomal complex / methylosome ...U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / histone pre-mRNA 3'end processing complex / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / protein methylation / U12-type spliceosomal complex / methylosome / 7-methylguanosine cap hypermethylation / U1 snRNP binding / pICln-Sm protein complex / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / telomerase holoenzyme complex / U2-type spliceosomal complex / U2-type precatalytic spliceosome / commitment complex / telomerase RNA binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / RNA Polymerase II Transcription Termination / U1 snRNP / U2-type prespliceosome / precatalytic spliceosome / mRNA Splicing - Minor Pathway / U5 snRNP / spliceosomal snRNP assembly / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / snRNP Assembly / SARS-CoV-2 modulates host translation machinery / nuclear body / enzyme binding / RNA binding / nucleoplasm / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kambach, C. / Walke, S. / Avis, J.M. / De La Fortelle, E. / Li, J. / Nagai, K. | ||||||
![]() | ![]() Title: Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs. Authors: Kambach, C. / Walke, S. / Young, R. / Avis, J.M. / de la Fortelle, E. / Raker, V.A. / Luhrmann, R. / Li, J. / Nagai, K. #1: ![]() Title: Structure and Assembly of the Spliceosomal Small Nuclear Ribonucleoprotein Particles Authors: Kambach, C. / Walke, S. / Nagai, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 211.4 KB | Display | ![]() |
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PDB format | ![]() | 171.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 558.2 KB | Display | ![]() |
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Full document | ![]() | 594.3 KB | Display | |
Data in XML | ![]() | 48.8 KB | Display | |
Data in CIF | ![]() | 65.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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5 | ![]()
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6 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Details | THE BIOLOGICALLY ACTIVE UNIT IS ONE D3B HETERODIMER, REPRESENTED BY THE PAIRS OF CHAINS A+B, C+D ETC. THE HETERODIMERS ARRANGE IN TWO HEXAMERIC RINGS WITH ALTERNATING D3 AND B SUBUNITS. THE TWO RINGS CONTACT EACH OTHER VIA A PARALLEL BETA-STRAND - BETA-STRAND INTERACTION. |
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Components
#1: Protein | Mass: 8491.800 Da / Num. of mol.: 6 / Fragment: SM MOTIF / Mutation: S66C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Description: N-TERMINAL HIS6 TAG CLEAVED OFF,TRUNCATED AT POSITION 75 Cellular location: NUCLEUS / Plasmid: PQE30 / Cellular location (production host): CYTOPLASM / Production host: ![]() ![]() #2: Protein | Mass: 10471.370 Da / Num. of mol.: 6 / Fragment: SM MOTIF / Source method: isolated from a natural source Details: POLYCISTRONIC COEXPRESSION VECTOR WITH SM D3. TRUNCATED AT POSITION 91. Source: (natural) ![]() #3: Chemical | ChemComp-GOL / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.1 % | ||||||||||||||||||||
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Crystal grow | pH: 8.5 / Details: pH 8.50 | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: AREA DETECTOR / Details: MIRRORS |
Radiation | Monochromator: SI CRYSTALS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99 Å / Relative weight: 1 |
Reflection | Resolution: 2→25.2 Å / Num. obs: 89816 / % possible obs: 99.2 % / Redundancy: 4.8 % / Biso Wilson estimate: 24.7 Å2 / Rsym value: 0.093 / Net I/σ(I): 6.1 |
Reflection shell | Resolution: 2→2.2 Å / Redundancy: 3.6 % / Mean I/σ(I) obs: 1.5 / Rsym value: 0.45 / % possible all: 98.8 |
Reflection | *PLUS Rmerge(I) obs: 0.093 |
Reflection shell | *PLUS % possible obs: 98.8 % / Rmerge(I) obs: 0.45 |
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Processing
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Refinement | Method to determine structure: ![]() Details: SIDECHAINS IN REGIONS OF POORLY DEFINED DENSITY (PARTICULARLY IN LOOPS) WERE MODELLED. OCCUPANCIES HAVE BEEN BEEN ARBITRARILY SET TO 0.10.
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Displacement parameters | Biso mean: 32 Å2
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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