positive regulation of interleukin-1 alpha production / induction of positive chemotaxis / CD4 receptor binding / Flemming body / leukocyte chemotaxis / Other interleukin signaling / regulation of calcium ion transport / positive regulation of interleukin-12 production / cytokine activity / positive regulation of inflammatory response ...positive regulation of interleukin-1 alpha production / induction of positive chemotaxis / CD4 receptor binding / Flemming body / leukocyte chemotaxis / Other interleukin signaling / regulation of calcium ion transport / positive regulation of interleukin-12 production / cytokine activity / positive regulation of inflammatory response / positive regulation of interleukin-6 production / nuclear speck / immune response / focal adhesion / extracellular space / extracellular region / plasma membrane / cytosol Similarity search - Function
Mass: 13394.903 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell: CD8+ T CELLS / Cell line: BL21 / Cellular location: EXTRACELLULAR / Plasmid: PET16B / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: Q14005
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
NOESY
1
2
1
TOCSY
NMR details
Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C-, 15N- LABELED INTERLEUKIN 16.
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Sample preparation
Details
Contents: WATER
Sample conditions
Ionic strength: 50 mM NACL / pH: 7.4 / Pressure: NORMAL / Temperature: 300 K
Crystal grow
*PLUS
Method: other / Details: NMR
-
NMR measurement
NMR spectrometer
Type: Bruker DMX750 / Manufacturer: Bruker / Model: DMX750 / Field strength: 750 MHz
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Processing
Software
Name
Version
Classification
X-PLOR
3.1
modelbuilding
X-PLOR
3.1
refinement
X-PLOR
3.1
phasing
NMR software
Name
Version
Developer
Classification
X-PLOR
3.1
BRUNGER
refinement
CCNMR
structuresolution
Refinement
Method: simulated annealing / Software ordinal: 1
NMR ensemble
Conformer selection criteria: LEAST RESTRAINT VIOLATION, LEAST TOTAL ENERGY Conformers calculated total number: 100 / Conformers submitted total number: 20
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