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- PDB-4bwo: The FedF adhesin from entrrotoxigenic Escherichia coli is a sulfa... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4bwo | ||||||
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Title | The FedF adhesin from entrrotoxigenic Escherichia coli is a sulfate- binding lectin | ||||||
![]() | F18 FIMBRIAL ADHESIN AC | ||||||
![]() | CELL ADHESION / GLYCAN ARRAY / ENTEROTOXIGENIC | ||||||
Function / homology | Jelly Rolls - #1210 / Jelly Rolls / Sandwich / Mainly Beta / BROMIDE ION / F18 fimbrial adhesin AC![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lonardi, E. / Moonens, K. / Buts, L. / de Boer, A.R. / Olsson, J.D.M. / Weiss, M.S. / Fabre, E. / Guerardel, Y. / Deelder, A.M. / Oscarson, S. ...Lonardi, E. / Moonens, K. / Buts, L. / de Boer, A.R. / Olsson, J.D.M. / Weiss, M.S. / Fabre, E. / Guerardel, Y. / Deelder, A.M. / Oscarson, S. / Wuhrer, M. / Bouckaert, J. | ||||||
![]() | ![]() Title: Structural Insight in Histo-Blood Group Binding by the F18 Fimbrial Adhesin Fedf. Authors: Moonens, K. / Bouckaert, J. / Coddens, A. / Tran, T. / Panjikar, S. / De Kerpel, M. / Cox, E. / Remaut, H. / De Greve, H. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2006 Title: N-Terminal Truncation Enables Crystallization of the Receptor-Binding Domain of the Fedf Bacterial Adhesin. Authors: De Kerpel, M. / Van Molle, I. / Brys, L. / Wyns, L. / De Greve, H. / Bouckaert, J. | ||||||
History |
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Remark 0 | THIS ENTRY 4BWO REFLECTS AN ALTERNATIVE MODELING OF THE ORIGINAL STRUCTURAL DATA (R4B4PSF) ...THIS ENTRY 4BWO REFLECTS AN ALTERNATIVE MODELING OF THE ORIGINAL STRUCTURAL DATA (R4B4PSF) DETERMINED BY AUTHORS OF THE PDB ENTRY 4B4P: K.MOONENS,J.BOUCKAERT,A.CODDENS,T.TRAN,S.PANJIKAR,M.DE KERPEL,E.COX, H.REMAUT,H.DE GREVE |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74.2 KB | Display | ![]() |
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PDB format | ![]() | 60 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.5 KB | Display | ![]() |
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Full document | ![]() | 451.8 KB | Display | |
Data in XML | ![]() | 16.6 KB | Display | |
Data in CIF | ![]() | 23.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16311.161 Da / Num. of mol.: 2 / Fragment: LECTIN DOMAIN, RESIDUES 35-185 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-BR / #3: Chemical | #4: Water | ChemComp-HOH / | Nonpolymer details | SULFATE ION (SO4): SULPHATE IONS BOUND OUTSIDE THE SUGAR BINDING SITE BROMIDE ION (BR): BROMINE TO ...SULFATE ION (SO4): SULPHATE IONS BOUND OUTSIDE THE SUGAR BINDING SITE BROMIDE ION (BR): BROMINE TO DETERMINE STRUCTURE USING MAD METHOD | Sequence details | FRAGMENT ENCOMPASSE | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.62 % / Description: AUTHOR USED THE SF DATA FROM ENTRY 4B4P. |
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Crystal grow | Details: 0.17 M AMMONIUM SULFATE, 25.5% (W/V) PEG 4000 AND 15% (V/V) GLYCEROL |
-Data collection
Diffraction source | Source: ![]() |
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Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Biso Wilson estimate: 18.14 Å2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.8→19.108 Å / SU ML: 0.17 / σ(F): 1.36 / Phase error: 19.1 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 1 Å2 / ksol: 1 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→19.108 Å
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Refine LS restraints |
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LS refinement shell |
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