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- PDB-1hzm: STRUCTURE OF ERK2 BINDING DOMAIN OF MAPK PHOSPHATASE MKP-3: STRUC... -

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Entry
Database: PDB / ID: 1hzm
TitleSTRUCTURE OF ERK2 BINDING DOMAIN OF MAPK PHOSPHATASE MKP-3: STRUCTURAL INSIGHTS INTO MKP-3 ACTIVATION BY ERK2
ComponentsDUAL SPECIFICITY PROTEIN PHOSPHATASE 6
KeywordsHYDROLASE
Function / homology
Function and homology information


regulation of heart growth / MAP kinase tyrosine phosphatase activity / protein tyrosine/threonine phosphatase activity / MAP kinase tyrosine/serine/threonine phosphatase activity / response to nitrosative stress / protein tyrosine/serine/threonine phosphatase activity / Signaling by MAPK mutants / RAF-independent MAPK1/3 activation / ERKs are inactivated / response to growth factor ...regulation of heart growth / MAP kinase tyrosine phosphatase activity / protein tyrosine/threonine phosphatase activity / MAP kinase tyrosine/serine/threonine phosphatase activity / response to nitrosative stress / protein tyrosine/serine/threonine phosphatase activity / Signaling by MAPK mutants / RAF-independent MAPK1/3 activation / ERKs are inactivated / response to growth factor / negative regulation of MAPK cascade / myosin phosphatase activity / protein-serine/threonine phosphatase / peptidyl-tyrosine dephosphorylation / ERK1 and ERK2 cascade / protein-tyrosine-phosphatase / negative regulation of protein phosphorylation / protein tyrosine phosphatase activity / response to organic cyclic compound / negative regulation of ERK1 and ERK2 cascade / Negative regulation of MAPK pathway / MAPK cascade / cell differentiation / response to xenobiotic stimulus / positive regulation of apoptotic process / nucleoplasm / cytoplasm / cytosol
Similarity search - Function
Mitogen-activated protein (MAP) kinase phosphatase / Rhodanese-like domain / Oxidized Rhodanese; domain 1 / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Rhodanese Homology Domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Rhodanese-like domain ...Mitogen-activated protein (MAP) kinase phosphatase / Rhodanese-like domain / Oxidized Rhodanese; domain 1 / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Rhodanese Homology Domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Rhodanese-like domain / Rhodanese domain profile. / Rhodanese-like domain superfamily / Rhodanese-like domain / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Protein-tyrosine phosphatase-like / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Dual specificity protein phosphatase 6
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR
AuthorsFarooq, A. / Zhou, M.-M.
CitationJournal: Mol.Cell / Year: 2001
Title: Solution structure of ERK2 binding domain of MAPK phosphatase MKP-3: structural insights into MKP-3 activation by ERK2.
Authors: Farooq, A. / Chaturvedi, G. / Mujtaba, S. / Plotnikova, O. / Zeng, L. / Dhalluin, C. / Ashton, R. / Zhou, M.M.
History
DepositionJan 25, 2001Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 25, 2002Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: DUAL SPECIFICITY PROTEIN PHOSPHATASE 6


Theoretical massNumber of molelcules
Total (without water)17,4251
Polymers17,4251
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein DUAL SPECIFICITY PROTEIN PHOSPHATASE 6 / MITOGEN-ACTIVATED PROTEIN KINASE PHOSPHATASE 3 / MAP KINASE PHOSPHATASE 3 / MKP-3


Mass: 17424.748 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-154
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: PET15B / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3)
References: UniProt: Q16828, protein-tyrosine-phosphatase, protein-serine/threonine phosphatase

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experimentType: 1H/15N-HSQC, HNCA, HN(CO)CA, HN(CA)CB, HN(COCA)CB, (H)C(CO)NH-TOCSY, 1H/15N-TOCSY, 1H/15N-NOESY, (H)CCH-TOCSY, 1H/13C-NOESY, HNHA-J

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Sample preparation

DetailsContents: UREA, NACL, IMIDAZOLE, DTT
Sample conditionsIonic strength: 200mM / pH: 6.0 / Pressure: 1 atm / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker DRXBrukerDRX6001
Bruker DRXBrukerDRX5002

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Processing

NMR software
NameClassification
XPLOR-ARIAstructure solution
XPLOR-ARIArefinement
NMR ensembleConformers submitted total number: 1

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