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Yorodumi- PDB-1hwg: 1:2 COMPLEX OF HUMAN GROWTH HORMONE WITH ITS SOLUBLE BINDING PROTEIN -
+Open data
-Basic information
Entry | Database: PDB / ID: 1hwg | ||||||
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Title | 1:2 COMPLEX OF HUMAN GROWTH HORMONE WITH ITS SOLUBLE BINDING PROTEIN | ||||||
Components |
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Keywords | COMPLEX (HORMONE/RECEPTOR) / CYTOKINE / HORMONE / RECEPTOR / HEMATOPOIETIC / COMPLEX (HORMONE-RECEPTOR) / COMPLEX (HORMONE-RECEPTOR) complex | ||||||
Function / homology | Function and homology information growth hormone receptor activity / regulation of response to nutrient levels / growth hormone activity / growth hormone receptor complex / bone maturation / prolactin receptor binding / positive regulation of growth / taurine metabolic process / animal organ development / cell surface receptor signaling pathway via STAT ...growth hormone receptor activity / regulation of response to nutrient levels / growth hormone activity / growth hormone receptor complex / bone maturation / prolactin receptor binding / positive regulation of growth / taurine metabolic process / animal organ development / cell surface receptor signaling pathway via STAT / response to gravity / cartilage development involved in endochondral bone morphogenesis / positive regulation of multicellular organism growth / hormone metabolic process / positive regulation of D-glucose transmembrane transport / proline-rich region binding / positive regulation of insulin-like growth factor receptor signaling pathway / growth hormone receptor binding / growth hormone receptor signaling pathway / response to food / response to cycloheximide / growth factor binding / Prolactin receptor signaling / cytokine binding / peptide hormone binding / cell surface receptor signaling pathway via JAK-STAT / regulation of multicellular organism growth / Synthesis, secretion, and deacylation of Ghrelin / growth hormone receptor signaling pathway via JAK-STAT / positive regulation of tyrosine phosphorylation of STAT protein / response to glucocorticoid / Growth hormone receptor signaling / cellular response to hormone stimulus / hormone-mediated signaling pathway / SH2 domain binding / response to interleukin-1 / insulin-like growth factor receptor signaling pathway / response to nutrient levels / cytokine activity / endosome lumen / positive regulation of cell differentiation / positive regulation of receptor signaling pathway via JAK-STAT / growth factor activity / positive regulation of MAP kinase activity / hormone activity / receptor internalization / cytokine-mediated signaling pathway / cytoplasmic ribonucleoprotein granule / cellular response to insulin stimulus / endocytosis / positive regulation of peptidyl-tyrosine phosphorylation / response to estradiol / protein phosphatase binding / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / receptor complex / external side of plasma membrane / neuronal cell body / lipid binding / protein kinase binding / cell surface / protein homodimerization activity / extracellular space / extracellular region / identical protein binding / membrane / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Sundstrom, S.M. / Lundqvist, T. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1996 Title: Crystal structure of an antagonist mutant of human growth hormone, G120R, in complex with its receptor at 2.9 A resolution. Authors: Sundstrom, M. / Lundqvist, T. / Rodin, J. / Giebel, L.B. / Milligan, D. / Norstedt, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hwg.cif.gz | 124.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hwg.ent.gz | 98.9 KB | Display | PDB format |
PDBx/mmJSON format | 1hwg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1hwg_validation.pdf.gz | 381.2 KB | Display | wwPDB validaton report |
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Full document | 1hwg_full_validation.pdf.gz | 392.2 KB | Display | |
Data in XML | 1hwg_validation.xml.gz | 13.4 KB | Display | |
Data in CIF | 1hwg_validation.cif.gz | 20.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hw/1hwg ftp://data.pdbj.org/pub/pdb/validation_reports/hw/1hwg | HTTPS FTP |
-Related structure data
Related structure data | 1hwhC 2hhr S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 22151.992 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: P01241 | ||
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#2: Protein | Mass: 27281.641 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR DOMAIN Source method: isolated from a genetically manipulated source Details: ONE HORMONE WITH TWO RECEPTOR MOLECULES / Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: P10912 #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50 % | ||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: THE PROTEIN COMPLEX WAS CRYSTALLISED AT PH 6.5 USING 45% (W/V) LISO4 WITH 2% PEG-500 DME AND SOAKED TO PH 5.5 PRIOR TO DATA COLLECTION | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 6.25 / Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 1, 1994 |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 2.5 Å / Num. obs: 25222 / % possible obs: 94.3 % / Observed criterion σ(I): 2 / Redundancy: 4.6 % / Rsym value: 0.097 / Net I/σ(I): 9 |
Reflection shell | Resolution: 2.5→2.7 Å / Redundancy: 2.3 % / Mean I/σ(I) obs: 2.7 / Rsym value: 0.313 / % possible all: 81.2 |
Reflection | *PLUS Lowest resolution: 20 Å / Rmerge(I) obs: 0.097 |
Reflection shell | *PLUS Rmerge(I) obs: 0.313 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2HHR 2hhr Resolution: 2.5→20 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 30.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.7 Å / Total num. of bins used: 6 / % reflection obs: 81.2 % |