[English] 日本語
Yorodumi- PDB-1hb8: Structure of bovine Acyl-CoA binding protein in tetragonal crysta... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1hb8 | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of bovine Acyl-CoA binding protein in tetragonal crystal form | ||||||
Components | ACYL-COA BINDING PROTEIN | ||||||
Keywords | ACYL-COENZYME A BINDING PROTEIN / ACYL-COA / BINDING PROTEIN | ||||||
Function / homology | Function and homology information Mitochondrial Fatty Acid Beta-Oxidation / fatty-acyl-CoA binding / fatty acid metabolic process / Golgi apparatus / endoplasmic reticulum Similarity search - Function | ||||||
Biological species | BOS TAURUS (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Zou, J.Y. / Kleywegt, G.J. / Bergfors, T. / Knudsen, J. / Jones, T.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: Binding Site Differences Revealed by Crystal Structures of Plasmodium Falciparum and Bovine Acyl-Coa Binding Protein Authors: Van Aalten, D.M.F. / Milne, K.G. / Zou, J.Y. / Kleywegt, G.J. / Bergfors, T. / Ferguson, M.A.J. / Knudsen, J. / Jones, T.A. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1hb8.cif.gz | 67 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1hb8.ent.gz | 51.3 KB | Display | PDB format |
PDBx/mmJSON format | 1hb8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hb/1hb8 ftp://data.pdbj.org/pub/pdb/validation_reports/hb/1hb8 | HTTPS FTP |
---|
-Related structure data
Related structure data | 1hb6C 1hbkC 2abdS S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
2 |
| ||||||||||||
3 |
| ||||||||||||
Unit cell |
| ||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
|
-Components
#1: Protein | Mass: 9931.289 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) BOS TAURUS (cattle) / References: UniProt: P07107 #2: Chemical | #3: Water | ChemComp-HOH / | Compound details | MAY FUNCTION AS AN INTRACELLULAR CARRIER OF ACYL-COA ESTERS. IT ALSO DISPLACES DIAZEPAM FROM THE ...MAY FUNCTION AS AN INTRACELLU | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 50.15 % |
---|---|
Crystal grow | pH: 7.5 Details: 25% PEG5000 MME, 40MM NI(SO4)2, 40MM HEPES PH7.5, pH 7.50 |
-Data collection
Diffraction | Mean temperature: 173 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7A / Wavelength: 0.928 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 15, 1995 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.928 Å / Relative weight: 1 |
Reflection | Resolution: 2→40 Å / Num. obs: 18831 / % possible obs: 96.5 % / Redundancy: 2.7 % / Biso Wilson estimate: 20.7 Å2 / Rmerge(I) obs: 0.066 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.292 / Mean I/σ(I) obs: 3.6 / % possible all: 98.8 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2ABD Resolution: 2→19.13 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1235568.81 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 54.4456 Å2 / ksol: 0.384682 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.9 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→19.13 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2→2.09 Å / Rfactor Rfree error: 0.029 / Total num. of bins used: 8
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
|