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- PDB-1h9c: NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1h9c | ||||||
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Title | NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli. | ||||||
![]() | PTS SYSTEM, CHITOBIOSE-SPECIFIC IIB COMPONENT | ||||||
![]() | TRANSFERASE / ENZYME IIB-CHITOBIOSE / PHOSPHOTRANSFERASE SYSTEM / SUGAR TRANSPORT / PHOSPHORYLATION / IIB- CELLOBIOSE | ||||||
Function / homology | ![]() protein-Npi-phosphohistidine-N,N'-diacetylchitobiose phosphotransferase / protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity / N,N'-diacetylchitobiose import / protein-N(PI)-phosphohistidine-sugar phosphotransferase activity / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / kinase activity / phosphorylation / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS, SIMULATED ANNEALING | ||||||
![]() | Ab, E. / Schuurman-Wolters, G.K. / Nijlant, D. / Dijkstra, K. / Saier, M.H. / Robillard, G.T. / Scheek, R.M. | ||||||
![]() | ![]() Title: NMR Structure of Cysteinyl-Phosphorylated Enzyme Iib of the N,N'-Diacetylchitobiose Specific Phosphoenolpyruvate-Dependentphosphotransferase System of Escherichia Coli Authors: Ab, E. / Schuurman-Wolters, G.K. / Nijlant, D. / Dijkstra, K. / Saier, M.H. / Robillard, G.T. / Scheek, R.M. #1: ![]() Title: The NMR Side-Chain Assignments and Solution Structure of Enzyme Iibcellobiose of the Phosphoenolpyruvate-Dependent Phosphotransferase System of Escherichia Coli Authors: Ab, E. / Schuurman-Wolters, G. / Reizer, J. / Saier, M.H. / Dijkstra, K. / Scheek, R.M. / Robillard, G.T. #2: Journal: Protein Sci. / Year: 1994 Title: Enzyme Iibcellobiose of the Phosphoenol-Pyruvate-Dependent Phosphotransferase System of Escherichia Coli: Backbone Assignment and Secondary Structure Determined by Three-Dimensional NMR Spectroscopy Authors: Ab, E. / Schuurman-Wolters, G.K. / Saier, M.H. / Reizer, J. / Jacuinod, M. / Roepstorff, P. / Dijkstra, K. / Scheek, R.M. / Robillard, G.T. #3: Journal: Genetics / Year: 1990 Title: Characterization and Nucleotide Sequence of the Cryptic Cel Operon of Escherichia Coli K12 Authors: Parker, L.L. / Hall, B.G. #4: Journal: Res.Microbiol. / Year: 1990 Title: The Cellobiose Permease of Escherichia Coli Consists of Three Proteins and is Homologous to the Lactose Permease of Staphylococcus Aureus Authors: Reizer, J. / Reizer, A. / Saier Jr, M.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 292.6 KB | Display | ![]() |
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PDB format | ![]() | 253 KB | Display | ![]() |
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-Validation report
Summary document | ![]() | 362.6 KB | Display | ![]() |
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Full document | ![]() | 400.1 KB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Data in CIF | ![]() | 25.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 11519.493 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: PHOSPHORYLATION AT RESIDUE CYS10 / Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P17409, UniProt: P69795*PLUS, protein-Npi-phosphohistidine-sugar phosphotransferase |
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Compound details | COMPONENT OF THE PHOSPHOENO |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: NOESY |
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Sample preparation
Sample conditions | pH: 7 / Pressure: 1 atm / Temperature: 295 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS, SIMULATED ANNEALING Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | |||||||||||||||
NMR ensemble | Conformer selection criteria: TARGET-FUNCTION, R-FACTOR, NUMBER OF VIOLATIONS Conformers calculated total number: 33 / Conformers submitted total number: 9 |