- PDB-1h45: R210G N-TERMINAL LOBE HUMAN LACTOFERRIN -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 1h45
タイトル
R210G N-TERMINAL LOBE HUMAN LACTOFERRIN
要素
LACTOFERRIN
キーワード
METAL TRANSPORT / IRON TRANSPORT / METAL BINDING
機能・相同性
機能・相同性情報
negative regulation by host of viral process / membrane destabilizing activity / Mtb iron assimilation by chelation / phagocytic vesicle lumen / positive regulation of toll-like receptor 4 signaling pathway / Metal sequestration by antimicrobial proteins / negative regulation of viral process / negative regulation of cysteine-type endopeptidase activity / negative regulation of tumor necrosis factor (ligand) superfamily member 11 production / negative regulation of single-species biofilm formation in or on host organism ...negative regulation by host of viral process / membrane destabilizing activity / Mtb iron assimilation by chelation / phagocytic vesicle lumen / positive regulation of toll-like receptor 4 signaling pathway / Metal sequestration by antimicrobial proteins / negative regulation of viral process / negative regulation of cysteine-type endopeptidase activity / negative regulation of tumor necrosis factor (ligand) superfamily member 11 production / negative regulation of single-species biofilm formation in or on host organism / positive regulation of bone mineralization involved in bone maturation / negative regulation of lipopolysaccharide-mediated signaling pathway / specific granule / negative regulation of osteoclast development / antifungal humoral response / positive regulation of chondrocyte proliferation / negative regulation of ATP-dependent activity / regulation of tumor necrosis factor production / bone morphogenesis / Antimicrobial peptides / cysteine-type endopeptidase inhibitor activity / negative regulation of viral genome replication / positive regulation of osteoblast proliferation / humoral immune response / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; セリンエンドペプチターゼ / positive regulation of osteoblast differentiation / regulation of cytokine production / protein serine/threonine kinase activator activity / ossification / innate immune response in mucosa / secretory granule / lipopolysaccharide binding / positive regulation of protein serine/threonine kinase activity / recycling endosome / specific granule lumen / antimicrobial humoral immune response mediated by antimicrobial peptide / tertiary granule lumen / positive regulation of NF-kappaB transcription factor activity / heparin binding / iron ion transport / antibacterial humoral response / positive regulation of canonical NF-kappaB signal transduction / defense response to Gram-negative bacterium / killing of cells of another organism / early endosome / iron ion binding / Amyloid fiber formation / serine-type endopeptidase activity / Neutrophil degranulation / negative regulation of apoptotic process / cell surface / protein-containing complex / proteolysis / DNA binding / extracellular space / extracellular exosome / extracellular region / nucleus / plasma membrane / cytoplasm 類似検索 - 分子機能
SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
IRON BINDING TRANSPORT PROTEINS THAT CAN BIND TWO FERRIC IRONS IN ASSOCIATION WITH THE BINDING OF ...IRON BINDING TRANSPORT PROTEINS THAT CAN BIND TWO FERRIC IRONS IN ASSOCIATION WITH THE BINDING OF AN ANION LIKE BICARBONATE. BELONGS TO THE TRANSFERRIN FAMILY. ENGINEERED MUTATION ARG 230 GLY CHAIN A
配列の詳細
REGARDING THE SEQUENCE MISMATCH AT RESIDUE 28, THE SEQUENCE THAT WAS USED IN THIS WORK IS FROM THE ...REGARDING THE SEQUENCE MISMATCH AT RESIDUE 28, THE SEQUENCE THAT WAS USED IN THIS WORK IS FROM THE SEQUENCING OF A CDNA FOR HUMAN LACTOFERRIN AND IS THAT GIVEN IN THE FOLLOWING PAPER: ANDERSON BF, BAKER HM, NORRIS GE, RICE DW AND BAKER EN. STRUCTURE OF HUMAN LACTOFERRIN: CRYSTALLOGRAPHIC ANALYSIS AND REFINEMENT AT 2.8 A RESOLUTION. J.MOL.BIOL. 1989. 209 711-734. THE MISMATCH AT 137 IS DERIVED FROM THE REMOVAL OF THE CARBOHYDRATE CHAIN FROM THE RECOMBINANT LACTOFERRIN USING A MIXTURE OF THE ENZYMES ENDOGLYCOSIDASE F AND PEPTIDE N-GLYCOSIDASE F FROM FLAVOBACTERIUM MENINGOSEPTICUM. THIS REACTION LEAVES A CARBOXYL GROUP AT THE GLYCOSYLATION SITE, SO RESIDUE 137 WAS BUILT AS ASP INSTEAD OF ASN.
-
実験情報
-
実験
実験
手法: X線回折 / 使用した結晶の数: 1
-
試料調製
結晶
マシュー密度: 2.74 Å3/Da / 溶媒含有率: 55.12 %
結晶化
pH: 8 / 詳細: HEPES, NACL, pH 8.00
結晶化
*PLUS
温度: 4 ℃ / pH: 8 / 手法: batch method
溶液の組成
*PLUS
ID
濃度
一般名
Crystal-ID
Sol-ID
詳細
化学式
1
28mg/ml
protein
1
1
2
20mM
HEPES
1
1
3
1M
1
1
pH8.0
NaCl
-
データ収集
回折
平均測定温度: 113 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RU200 / 波長: 1.5418
検出器
タイプ: RIGAKU IMAGE PLATE / 検出器: IMAGE PLATE
放射
モノクロメーター: GRAPHITE / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
解像度: 1.95→30 Å / Num. obs: 27857 / % possible obs: 94.2 % / 冗長度: 2.8 % / Biso Wilson estimate: 17.8 Å2 / Rmerge(I) obs: 0.053 / Net I/σ(I): 13.6
反射 シェル
解像度: 1.95→2.02 Å / Rmerge(I) obs: 0.146 / Mean I/σ(I) obs: 4.2 / % possible all: 60.4