+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1h10 | ||||||
---|---|---|---|---|---|---|---|
タイトル | HIGH RESOLUTION STRUCTURE OF THE PLECKSTRIN HOMOLOGY DOMAIN OF PROTEIN KINASE B/AKT BOUND TO INS(1,3,4,5)-TETRAKISPHOPHATE | ||||||
要素 | RAC-ALPHA SERINE/THREONINE KINASE | ||||||
キーワード | TRANSFERASE / SIGNALLING PROTEIN / PHOSPHOINOSITIDES / PROTEIN KINASE B / PLECKSTRIN / INOSITOL TETRAKISPHOPHATE / PHOSPHORYLATION / SERINE/THREONINE PROTEIN KINASE | ||||||
機能・相同性 | 機能・相同性情報 glycogen cell differentiation involved in embryonic placenta development / regulation of tRNA methylation / response to insulin-like growth factor stimulus / potassium channel activator activity / negative regulation of protein localization to lysosome / positive regulation of protein localization to endoplasmic reticulum / maintenance of protein location in mitochondrion / negative regulation of lymphocyte migration / cellular response to decreased oxygen levels / regulation of type B pancreatic cell development ...glycogen cell differentiation involved in embryonic placenta development / regulation of tRNA methylation / response to insulin-like growth factor stimulus / potassium channel activator activity / negative regulation of protein localization to lysosome / positive regulation of protein localization to endoplasmic reticulum / maintenance of protein location in mitochondrion / negative regulation of lymphocyte migration / cellular response to decreased oxygen levels / regulation of type B pancreatic cell development / AKT-mediated inactivation of FOXO1A / maternal placenta development / Negative regulation of the PI3K/AKT network / negative regulation of long-chain fatty acid import across plasma membrane / establishment of protein localization to mitochondrion / negative regulation of fatty acid beta-oxidation / AKT phosphorylates targets in the nucleus / regulation of glycogen biosynthetic process / cellular response to oxidised low-density lipoprotein particle stimulus / negative regulation of cilium assembly / positive regulation of I-kappaB phosphorylation / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / response to fluid shear stress / RUNX2 regulates genes involved in cell migration / positive regulation of organ growth / MTOR signalling / fibroblast migration / interleukin-18-mediated signaling pathway / positive regulation of sodium ion transport / mammary gland epithelial cell differentiation / negative regulation of endopeptidase activity / negative regulation of protein serine/threonine kinase activity / RAB GEFs exchange GTP for GDP on RABs / positive regulation of glucose metabolic process / positive regulation of endodeoxyribonuclease activity / response to growth factor / cellular response to granulocyte macrophage colony-stimulating factor stimulus / positive regulation of protein localization to cell surface / protein serine/threonine kinase inhibitor activity / negative regulation of leukocyte cell-cell adhesion / phosphatidylinositol-3,4-bisphosphate binding / peripheral nervous system myelin maintenance / sphingosine-1-phosphate receptor signaling pathway / positive regulation of fibroblast migration / cell migration involved in sprouting angiogenesis / response to growth hormone / anoikis / glycogen biosynthetic process / AKT phosphorylates targets in the cytosol / labyrinthine layer blood vessel development / execution phase of apoptosis / response to food / response to UV-A / regulation of myelination / regulation of postsynapse organization / regulation of neuron projection development / KSRP (KHSRP) binds and destabilizes mRNA / Regulation of TP53 Activity through Association with Co-factors / mammalian oogenesis stage / negative regulation of macroautophagy / CTLA4 inhibitory signaling / negative regulation of cGAS/STING signaling pathway / activation-induced cell death of T cells / negative regulation of Notch signaling pathway / behavioral response to pain / non-canonical NF-kappaB signal transduction / negative regulation of release of cytochrome c from mitochondria / Constitutive Signaling by AKT1 E17K in Cancer / apoptotic mitochondrial changes / CD28 dependent PI3K/Akt signaling / phosphatidylinositol-3,4,5-trisphosphate binding / Regulation of localization of FOXO transcription factors / positive regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of glycogen biosynthetic process / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / TOR signaling / Activation of BAD and translocation to mitochondria / positive regulation of blood vessel endothelial cell migration / cellular response to vascular endothelial growth factor stimulus / positive regulation of fat cell differentiation / canonical NF-kappaB signal transduction / positive regulation of G1/S transition of mitotic cell cycle / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / eNOS activation / positive regulation of lipid biosynthetic process / Cyclin E associated events during G1/S transition / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / Cyclin A:Cdk2-associated events at S phase entry / Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation / lipopolysaccharide-mediated signaling pathway / regulation of cell migration / negative regulation of protein ubiquitination / cellular response to epidermal growth factor stimulus / 14-3-3 protein binding / Regulation of TP53 Activity through Acetylation / positive regulation of TORC1 signaling / positive regulation of endothelial cell proliferation / striated muscle cell differentiation / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 類似検索 - 分子機能 | ||||||
生物種 | HOMO SAPIENS (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / DIRECT METHODS / 解像度: 1.4 Å | ||||||
データ登録者 | Thomas, C.C. / Deak, M. / Alessi, D.R. / Van Aalten, D.M.F. | ||||||
引用 | ジャーナル: Curr.Biol. / 年: 2002 タイトル: High Resolution Structure of the Pleckstrin Homology Domain of Protein Kinase B/Akt Bound to Phosphatidylinositol (3,4,5)-Trisphosphate 著者: Thomas, C.C. / Deak, M. / Alessi, D.R. / Van Aalten, D.M.F. | ||||||
履歴 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1h10.cif.gz | 71.7 KB | 表示 | PDBx/mmCIF形式 |
---|---|---|---|---|
PDB形式 | pdb1h10.ent.gz | 56.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1h10.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1h10_validation.pdf.gz | 856.4 KB | 表示 | wwPDB検証レポート |
---|---|---|---|---|
文書・詳細版 | 1h10_full_validation.pdf.gz | 857.9 KB | 表示 | |
XML形式データ | 1h10_validation.xml.gz | 10 KB | 表示 | |
CIF形式データ | 1h10_validation.cif.gz | 14.7 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/h1/1h10 ftp://data.pdbj.org/pub/pdb/validation_reports/h1/1h10 | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
単位格子 |
| ||||||||
Components on special symmetry positions |
|
-要素
#1: タンパク質 | 分子量: 14940.391 Da / 分子数: 1 / 断片: PLECKSTRIN HOMOLOGY DOMAIN, RESIDUES 1-123 / 由来タイプ: 組換発現 詳細: BOUND TO INS(1,3,4,5)-TETRAKISPHOPHATE, SELENOMETHIONINE DERIVATIVE 由来: (組換発現) HOMO SAPIENS (ヒト) / プラスミド: PGEX4T-1 / 発現宿主: ESCHERICHIA COLI (大腸菌) / 株 (発現宿主): BL21 参照: UniProt: P31749, 転移酵素; リンを含む基を移すもの; キナーゼ(アルコールにつなげるもの) |
---|---|
#2: 化合物 | ChemComp-4IP / |
#3: 水 | ChemComp-HOH / |
構成要素の詳細 | GENERAL PROTEIN KINASE, PHOSPHORYL |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
---|
-試料調製
結晶 | マシュー密度: 1.94 Å3/Da / 溶媒含有率: 36.07 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
結晶化 | pH: 4.2 詳細: 0.25M AMMONIUM ACETATE, 30% PEG 4000,0.1M SODIUM ACETATE (PH 4.6) | ||||||||||||||||||||||||||||||
結晶化 | *PLUS pH: 4.6 / 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
|
-データ収集
回折 | 平均測定温度: 100 K |
---|---|
放射光源 | 由来: シンクロトロン / サイト: SRS / ビームライン: PX14.2 / 波長: 0.9786 |
検出器 | タイプ: ADSC CCD / 検出器: CCD / 日付: 2002年1月15日 |
放射 | プロトコル: MAD / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9786 Å / 相対比: 1 |
反射 | 解像度: 1.4→15 Å / Num. obs: 21933 / % possible obs: 97.8 % / 冗長度: 3.8 % / Rmerge(I) obs: 0.055 / Net I/σ(I): 30.8 |
反射 シェル | 解像度: 1.4→1.45 Å / 冗長度: 2.6 % / Rmerge(I) obs: 0.231 / Mean I/σ(I) obs: 6.7 / % possible all: 83.3 |
反射 | *PLUS 最高解像度: 1.4 Å / 最低解像度: 15 Å / 冗長度: 3.8 % / Num. measured all: 83520 / Rmerge(I) obs: 0.055 |
反射 シェル | *PLUS 最高解像度: 1.4 Å / % possible obs: 83.3 % / 冗長度: 2.6 % / Rmerge(I) obs: 0.231 / Mean I/σ(I) obs: 6.7 |
-解析
ソフトウェア |
| |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 構造決定の手法: DIRECT METHODS / 解像度: 1.4→15 Å / Num. parameters: 11393 / Num. restraintsaints: 10950 / 交差検証法: FREE R-VALUE / σ(F): 0 / 立体化学のターゲット値: ENGH AND HUBER / 詳細: REFINEMENT USING CNS AND THEN SHELXL -97
| |||||||||||||||||||||||||||||||||
Refine analyze | Occupancy sum hydrogen: 943 / Occupancy sum non hydrogen: 1249.5 | |||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.4→15 Å
| |||||||||||||||||||||||||||||||||
拘束条件 |
| |||||||||||||||||||||||||||||||||
ソフトウェア | *PLUS 名称: SHELXL / バージョン: 97 / 分類: refinement | |||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor obs: 0.125 / Rfactor Rfree: 0.175 / Rfactor Rwork: 0.125 | |||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | |||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
|