[English] 日本語
![](img/lk-miru.gif)
- PDB-2uzs: A transforming mutation in the pleckstrin homology domain of AKT1... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2uzs | ||||||
---|---|---|---|---|---|---|---|
Title | A transforming mutation in the pleckstrin homology domain of AKT1 in cancer (AKT1-PH_E17K) | ||||||
![]() | RAC-alpha serine/threonine-protein kinase | ||||||
![]() | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() glycogen cell differentiation involved in embryonic placenta development / regulation of tRNA methylation / response to insulin-like growth factor stimulus / potassium channel activator activity / negative regulation of protein localization to lysosome / positive regulation of protein localization to endoplasmic reticulum / maintenance of protein location in mitochondrion / negative regulation of lymphocyte migration / cellular response to decreased oxygen levels / regulation of type B pancreatic cell development ...glycogen cell differentiation involved in embryonic placenta development / regulation of tRNA methylation / response to insulin-like growth factor stimulus / potassium channel activator activity / negative regulation of protein localization to lysosome / positive regulation of protein localization to endoplasmic reticulum / maintenance of protein location in mitochondrion / negative regulation of lymphocyte migration / cellular response to decreased oxygen levels / regulation of type B pancreatic cell development / AKT-mediated inactivation of FOXO1A / maternal placenta development / Negative regulation of the PI3K/AKT network / negative regulation of long-chain fatty acid import across plasma membrane / establishment of protein localization to mitochondrion / negative regulation of fatty acid beta-oxidation / regulation of glycogen biosynthetic process / AKT phosphorylates targets in the nucleus / cellular response to oxidised low-density lipoprotein particle stimulus / negative regulation of cilium assembly / positive regulation of I-kappaB phosphorylation / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / response to fluid shear stress / RUNX2 regulates genes involved in cell migration / positive regulation of organ growth / MTOR signalling / fibroblast migration / interleukin-18-mediated signaling pathway / positive regulation of sodium ion transport / mammary gland epithelial cell differentiation / negative regulation of endopeptidase activity / negative regulation of protein serine/threonine kinase activity / mammalian oogenesis stage / RAB GEFs exchange GTP for GDP on RABs / positive regulation of glucose metabolic process / positive regulation of endodeoxyribonuclease activity / positive regulation of protein localization to cell surface / protein serine/threonine kinase inhibitor activity / cellular response to granulocyte macrophage colony-stimulating factor stimulus / response to growth factor / activation-induced cell death of T cells / negative regulation of leukocyte cell-cell adhesion / phosphatidylinositol-3,4-bisphosphate binding / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Carpten, J.D. / Faber, A.L. / Horn, C. / Donoho, G.P. / Briggs, S.L. / Robbins, C.M. / Hostetter, G. / Boguslawski, S. / Moses, T.Y. / Savage, S. ...Carpten, J.D. / Faber, A.L. / Horn, C. / Donoho, G.P. / Briggs, S.L. / Robbins, C.M. / Hostetter, G. / Boguslawski, S. / Moses, T.Y. / Savage, S. / Uhlik, M. / Lin, A. / Du, J. / Qian, Y.W. / Zeckner, D.J. / Tucker-Kellogg, G. / Touchman, J. / Patel, K. / Mousses, S. / Bittner, M. / Schevitz, R. / Lai, M.H. / Blanchard, K.L. / Thomas, J.E. | ||||||
![]() | ![]() Title: A transforming mutation in the pleckstrin homology domain of AKT1 in cancer. Authors: Carpten, J.D. / Faber, A.L. / Horn, C. / Donoho, G.P. / Briggs, S.L. / Robbins, C.M. / Hostetter, G. / Boguslawski, S. / Moses, T.Y. / Savage, S. / Uhlik, M. / Lin, A. / Du, J. / Qian, Y.W. ...Authors: Carpten, J.D. / Faber, A.L. / Horn, C. / Donoho, G.P. / Briggs, S.L. / Robbins, C.M. / Hostetter, G. / Boguslawski, S. / Moses, T.Y. / Savage, S. / Uhlik, M. / Lin, A. / Du, J. / Qian, Y.W. / Zeckner, D.J. / Tucker-Kellogg, G. / Touchman, J. / Patel, K. / Mousses, S. / Bittner, M. / Schevitz, R. / Lai, M.H. / Blanchard, K.L. / Thomas, J.E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 41.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 27.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 2uzrC ![]() 1unqS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 14799.770 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-123 / Mutation: E17K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P31749, ![]() | ||
---|---|---|---|
#2: Chemical | ChemComp-4IP / | ||
#3: Water | ChemComp-HOH / ![]() | ||
Compound details | ENGINEEREDSequence details | E17K | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 1.64 Å3/Da / Density % sol: 25.27 % |
---|---|
Crystal grow![]() | Method: vapor diffusion, hanging drop / pH: 7.5 Details: GROWN FROM HANGING DROPS IN 0.1 M HEPES PH 7.5 AND 1.4 M SODIUM CITRATE, OR 0.1 M ACETATE PH 4.6, 0.2 M AMMONIUM ACETATE AND 15%-30% PEG 3350, |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jul 17, 2006 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.46→37.5 Å / Num. obs: 3647 / % possible obs: 97 % / Observed criterion σ(I): 2 / Redundancy: 2 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 13.5 |
Reflection shell | Resolution: 2.45→2.54 Å / Redundancy: 1.54 % / Rmerge(I) obs: 0.13 / Mean I/σ(I) obs: 9.5 / % possible all: 88.9 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1UNQ Resolution: 2.46→37.53 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.86 / SU B: 13.569 / SU ML: 0.3 / Cross valid method: THROUGHOUT / ESU R Free: 0.405 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.66 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.46→37.53 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|