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Yorodumi- PDB-1gmq: COMPLEX OF RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS WITH 2'-GM... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1gmq | ||||||
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Title | COMPLEX OF RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS WITH 2'-GMP AT 1.7 ANGSTROMS RESOLUTION | ||||||
Components | RIBONUCLEASE SA | ||||||
Keywords | HYDROLASE / GUANYLORIBONUCLEASE | ||||||
Function / homology | Function and homology information ribonuclease T1 activity / ribonuclease T1 / RNA endonuclease activity / lyase activity / RNA binding / extracellular region Similarity search - Function | ||||||
Biological species | Streptomyces aureofaciens (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.8 Å | ||||||
Authors | Sevcik, J. / Hill, C. / Dauter, Z. / Wilson, K. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.B / Year: 1991 Title: Continuation of the Rnase Sa Work After 1Sar and 2Sar Structures Authors: Sevcik, J. / Dodson, E.J. / Dodson, G.G. | ||||||
History |
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Remark 0 | THIS ENTRY 1GMQ REFLECTS AN ALTERNATIVE MODELING OF THE ORIGINAL STRUCTURAL DATA R1SARSF DETERMINED ...THIS ENTRY 1GMQ REFLECTS AN ALTERNATIVE MODELING OF THE ORIGINAL STRUCTURAL DATA R1SARSF DETERMINED BY AUTHORS OF THE PDB ENTRY 1SAR: AUTHOR J.SEVCIK,E.J.DODSON,G.G.DODSON | ||||||
Remark 200 | AUTHOR USED THE SF DATA FROM ENTRY 1SAR. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gmq.cif.gz | 55.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gmq.ent.gz | 42.6 KB | Display | PDB format |
PDBx/mmJSON format | 1gmq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1gmq_validation.pdf.gz | 427.1 KB | Display | wwPDB validaton report |
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Full document | 1gmq_full_validation.pdf.gz | 430.5 KB | Display | |
Data in XML | 1gmq_validation.xml.gz | 15.4 KB | Display | |
Data in CIF | 1gmq_validation.cif.gz | 23.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gm/1gmq ftp://data.pdbj.org/pub/pdb/validation_reports/gm/1gmq | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO A 27 AND PRO B 27 ARE CIS PROLINES. |
-Components
#1: Protein | Mass: 10584.531 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces aureofaciens (bacteria) / References: UniProt: P05798, EC: 3.1.27.3 #2: Chemical | ChemComp-SO4 / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.72 % | ||||||||||||
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Crystal grow | *PLUS pH: 7.2 / Method: vapor diffusion / Details: referred to Acta Cryst.B47.240-253 1991 | ||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.8 Å / Num. obs: 18996 / % possible obs: 99.3 % / Num. measured all: 71868 / Rmerge(I) obs: 0.033 |
-Processing
Software | Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.139 / Highest resolution: 1.8 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.8 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 10 Å / Num. reflection obs: 18895 / Rfactor obs: 0.139 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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