[English] 日本語
![](img/lk-miru.gif)
- PDB-1rsn: RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CY... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1rsn | ||||||
---|---|---|---|---|---|---|---|
Title | RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE | ||||||
![]() | RIBONUCLEASE SA | ||||||
![]() | HYDROLASE (GUANYLORIBONUCLEASE) | ||||||
Function / homology | ![]() ribonuclease T1 activity / ribonuclease T1 / RNA endonuclease activity / lyase activity / RNA binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sevcik, J. / Dauter, Z. / Lamzin, V.S. / Wilson, K.S. | ||||||
![]() | ![]() Title: Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate. Authors: Sevcik, J. / Zegers, I. / Wyns, L. / Dauter, Z. / Wilson, K.S. #1: ![]() Title: Complex of Ribonuclease from Streptomyces Aureofaciens with 2'-Gmp at 1.7 Angstroms Resolution Authors: Sevcik, J. / Hill, C.P. / Dauter, Z. / Wilson, K.S. #2: ![]() Title: Determination and Restrained Least-Squares Refinement of the Structure of Ribonuclease And its Complex with 3'-Guanylic Acid at 1.8 Angstroms Resolution Authors: Sevcik, J. / Dodson, E.J. / Dodson, G.G. #3: ![]() Title: Comparison of Active Sites of Some Microbial Ribonucleases: Structural Basis for Guanylic Specificity Authors: Sevcik, J. / Sanishvili, R.G. / Pavlovsky, A.G. / Polyakov, K.M. #4: ![]() Title: Amino Acid Sequence Determination of Guanyl-Specific Ribonuclease Sa from Streptomyces Aureofaciens Authors: Shlyapnikov, S.U. / Both, V. / Kulikov, V.A. / Dementiev, A.A. / Sevcik, J. / Zelinka, J. #5: ![]() Title: Exocellular Ribonuclease from Streptomyces Aureofaciens. I. Isolation and Purification Authors: Bacova, M. / Zelinkova, E. / Zelinka, J. #6: ![]() Title: Exocellular Ribonuclease from Streptomyces Aureofaciens. II. Properties and Specificity Authors: Bacova, M. / Zelinkova, E. / Zelinka, J. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 58.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 46.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 25 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: CIS PROLINE - PRO A 27 / 2: CIS PROLINE - PRO B 27 | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.96983, 0.24082, 0.03791), Vector: Details | THE ASYMMETRIC UNIT CONTAINS TWO CHEMICALLY IDENTICAL SUBUNITS WHICH ARE RELATED BY A NON-CRYSTALLOGRAPHIC SYMMETRY. MTRIX THE TRANSFORMATIONS PRESENTED ON MTRIX RECORDS BELOW DESCRIBE NON-CRYSTALLOGRAPHIC RELATIONSHIPS AMONG THE VARIOUS DOMAINS IN THIS ENTRY. APPLYING THE APPROPRIATE MTRIX TRANSFORMATION TO THE RESIDUES LISTED FIRST WILL YIELD APPROXIMATE COORDINATES FOR THE RESIDUES LISTED SECOND. APPLIED TO TRANSFORMED TO MTRIX RESIDUES RESIDUES RMSD M1 A 1 .. A 96 B 1 .. B 96 0.375 | |
-
Components
#1: Protein | Mass: 10584.531 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Chemical | ChemComp-SO4 / | #3: Chemical | #4: Water | ChemComp-HOH / | Compound details | SECONDARY STRUCTURE BOUNDARIES HAVE BEEN DETERMINED USING THE SS PROGRAM (V.S.LAMZIN, EMBL HAMBURG) ...SECONDARY STRUCTURE BOUNDARIES | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.72 % | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal | *PLUS Density % sol: 50 % | ||||||||||||||||||||
Crystal grow | *PLUS pH: 7.2 / Method: vapor diffusion | ||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
---|---|
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 1, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.95 Å / Relative weight: 1 |
Reflection | Redundancy: 3.4 % / Rmerge(I) obs: 0.034 |
Reflection | *PLUS Highest resolution: 2 Å / Num. obs: 13932 / % possible obs: 98.4 % / Num. measured all: 48382 / Rmerge(I) obs: 0.034 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2→10 Å / σ(F): 0 /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|