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- PDB-1gmr: COMPLEX OF RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS WITH 2'-GM... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1gmr | ||||||
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Title | COMPLEX OF RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS WITH 2'-GMP AT 1.7 ANGSTROMS RESOLUTION | ||||||
![]() | RIBONUCLEASE SA | ||||||
![]() | HYDROLASE(GUANYLORIBONUCLEASE) | ||||||
Function / homology | ![]() ribonuclease T1 activity / ribonuclease T1 / RNA endonuclease activity / lyase activity / RNA binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Sevcik, J. / Hill, C. / Dauter, Z. / Wilson, K. | ||||||
![]() | ![]() Title: Complex of ribonuclease from Streptomyces aureofaciens with 2'-GMP at 1.7 A resolution. Authors: Sevcik, J. / Hill, C.P. / Dauter, Z. / Wilson, K.S. #1: ![]() Title: Continuation of the Rnase Sa Work After 1Sar and 2Sar Structures Authors: Sevcik, J. / Dodson, E.J. / Dodson, G.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 56 KB | Display | ![]() |
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PDB format | ![]() | 44.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 16.3 KB | Display | |
Data in CIF | ![]() | 24 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO A 27 AND PRO B 27 ARE CIS PROLINES. |
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Components
#1: Protein | Mass: 10584.531 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() #2: Chemical | ChemComp-SO4 / | #3: Chemical | #4: Water | ChemComp-HOH / | Nonpolymer details | THERE TOO MANY WATER MOLECULES AT BONDING DISTANCE FROM ATOMS OF 2GP B. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.72 % | ||||||||||||
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Crystal grow | *PLUS pH: 7.2 / Method: vapor diffusion / Details: referred to Acta Cryst.B47.240-253 1991 | ||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.77 Å / Num. obs: 19476 / % possible obs: 96.3 % / Num. measured all: 56410 / Rmerge(I) obs: 0.042 |
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Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.77→10 Å /
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Refinement step | Cycle: LAST / Resolution: 1.77→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.77 Å / Lowest resolution: 10 Å / Num. reflection obs: 19319 / Rfactor obs: 0.146 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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