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- PDB-1lni: CRYSTAL STRUCTURE ANALYSIS OF A RIBONUCLEASE FROM STREPTOMYCES AU... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1lni | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF A RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS AT ATOMIC RESOLUTION (1.0 A) | ||||||
![]() | GUANYL-SPECIFIC RIBONUCLEASE SA | ||||||
![]() | HYDROLASE / Ribonuclease Sa | ||||||
Function / homology | ![]() ribonuclease T1 activity / ribonuclease T1 / RNA endonuclease activity / lyase activity / RNA binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sevcik, J. / Lamzin, V.S. / Dauter, Z. / Wilson, K.S. | ||||||
![]() | ![]() Title: Atomic resolution data reveal flexibility in the structure of RNase Sa. Authors: Sevcik, J. / Lamzin, V.S. / Dauter, Z. / Wilson, K.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.1 KB | Display | ![]() |
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PDB format | ![]() | 89.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.7 KB | Display | ![]() |
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Full document | ![]() | 447.6 KB | Display | |
Data in XML | ![]() | 17.5 KB | Display | |
Data in CIF | ![]() | 27.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1rggS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10582.492 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 48 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: AREA DETECTOR / Date: Aug 25, 1995 / Details: mirrors |
Radiation | Monochromator: triangular asymmetrically cut Si(111)or Ge(111) Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1→19.5 Å / Num. obs: 101141 / % possible obs: 97.5 % / Observed criterion σ(I): -3 / Redundancy: 3.9 % / Biso Wilson estimate: 7.1 Å2 / Rmerge(I) obs: 0.042 / Net I/σ(I): 28.7 |
Reflection shell | Resolution: 1→1.02 Å / Rmerge(I) obs: 0.539 / Mean I/σ(I) obs: 2.4 / Num. unique all: 4660 / % possible all: 91.1 |
Reflection | *PLUS Num. obs: 101172 |
Reflection shell | *PLUS % possible obs: 91.1 % |
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Processing
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Refinement | Method to determine structure: STARTING MODEL WAS USED WITHOUT molecular replacement Starting model: PDB ENTRY 1RGG Resolution: 1→19.5 Å / Stereochemistry target values: Engh & Huber /
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Refinement step | Cycle: LAST / Resolution: 1→19.5 Å
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Software | *PLUS Name: SHELXL / Version: 93 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.119 / Rfactor Rwork: 0.119 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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