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- PDB-1ggw: CDC4P FROM SCHIZOSACCHAROMYCES POMBE -

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Basic information

Entry
Database: PDB / ID: 1ggw
TitleCDC4P FROM SCHIZOSACCHAROMYCES POMBE
ComponentsPROTEIN (CDC4P)
KeywordsCYTOKINE / LIGHT CHAIN / CYTOKINESIS / CELL CYCLE / EF-HAND
Function / homology
Function and homology information


RHO GTPases activate PAKs / medial cortical node / mitotic actomyosin contractile ring / mitotic actomyosin contractile ring assembly / division septum assembly / myosin II complex / cell division site / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
EF-hand / : / Recoverin; domain 1 / EF-hand domain pair / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Myosin regulatory light chain cdc4
Similarity search - Component
Biological speciesSchizosaccharomyces pombe (fission yeast)
MethodSOLUTION NMR / simulated annealing
AuthorsSlupsky, C.M. / Hemmingsen, S.M. / McIntosh, L.P.
Citation
Journal: J.Biol.Chem. / Year: 2001
Title: Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe.
Authors: Slupsky, C.M. / Desautels, M. / Huebert, T. / Zhao, R. / Hemmingsen, S.M. / McIntosh, L.P.
#1: Journal: J.Biol.Chem. / Year: 2001
Title: Cdc4p, a Contractile Ring Protein Essential for Cytokinesis in Schizosaccharomyces pombe, Interacts with a Phosphatidylinositol 4-Kinase
Authors: Desautels, M. / Den Haese, J.P. / Slupsky, C.M. / McIntosh, L.P. / Hemmingsen, S.M.
History
DepositionSep 25, 2000Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 21, 2001Provider: repository / Type: Initial release
Revision 1.1Apr 26, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4Dec 27, 2023Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: PROTEIN (CDC4P)


Theoretical massNumber of molelcules
Total (without water)15,5431
Polymers15,5431
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)26 / 50LEAST RESTRAINT VIOLATION
RepresentativeModel #5

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Components

#1: Protein PROTEIN (CDC4P) / EF-HAND PROTEIN / MYOSIN LIGHT CHAIN


Mass: 15543.479 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Schizosaccharomyces pombe (fission yeast)
Strain: WILD-TYPE 972 / Cellular location: CYTOPLASM / Plasmid: PT7-7 / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Gene (production host): CDC4 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q09196

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1113D HN(CA)CB
1213D CBCA(CO)NH
1313D C(CO)NH
1413D (H)CCH-TOCSY
1513D-15N-TOCSY-HSQC
1613D-15N-NOESY-HSQC
1713D HNHB
1812D-NOESY
1912D-COSY
11013D HNHA
11113D-13C/15N-NOESY-HSQC
11214D-13C/13C-HMQC-NOESY-HMQC
NMR detailsText: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C,15N-LABELED CDC4P, AND 15N-LABELED CDC4P

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Sample preparation

DetailsContents: H2O / D2O
Sample conditionsIonic strength: 100 mM KCL / pH: 6.5 / Pressure: 1.0 ATM / Temperature: 303 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 500 MHz

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Processing

NMR software
NameVersionDeveloperClassification
X-PLOR3.8BRUNGERrefinement
X-PLORstructure solution
RefinementMethod: simulated annealing / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JOURNAL CITATION ABOVE
NMR ensembleConformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 50 / Conformers submitted total number: 26

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