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Open data
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Basic information
Entry | Database: PDB / ID: 1gao | ||||||
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Title | CRYSTAL STRUCTURE OF THE L44S MUTANT OF FERREDOXIN I | ||||||
![]() | FERREDOXIN I | ||||||
![]() | ELECTRON TRANSPORT / Iron-sulfur clusters / ferredoxin | ||||||
Function / homology | ![]() 3 iron, 4 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / electron transfer activity / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Stout, C.D. / Burgess, B.K. / Prasad, G.S. / Sridhar, V. / Jung, Y.S. | ||||||
![]() | ![]() Title: Azotobacter vinelandii ferredoxin I: a sequence and structure comparison approach to alteration of [4Fe-4S]2+/+ reduction potential. Authors: Chen, K. / Jung, Y.S. / Bonagura, C.A. / Tilley, G.J. / Prasad, G.S. / Sridhar, V. / Armstrong, F.A. / Stout, C.D. / Burgess, B.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 106.2 KB | Display | ![]() |
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PDB format | ![]() | 80.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422 KB | Display | ![]() |
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Full document | ![]() | 431 KB | Display | |
Data in XML | ![]() | 10 KB | Display | |
Data in CIF | ![]() | 16.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1g3oC ![]() 1g6bC ![]() 7fd1S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12033.450 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Chemical | ChemComp-SF4 / #3: Chemical | ChemComp-F3S / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.45 Å3/Da / Density % sol: 64.36 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / pH: 7.5 Details: Ammonium sulfate, Lithium sulfate, Tris buffer, pH 7.5, VAPOR DIFFUSION, temperature 277K | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 2 ℃ / pH: 7.4 / Details: Shen, B., (1994) J. Biol. Chem., 269, 8564. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 17, 2000 / Details: SSRL beam line 7-1 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 55252 / Num. obs: 30964 / % possible obs: 93.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 1.8 % / Rmerge(I) obs: 0.136 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7FD1 Resolution: 2.2→50 Å / Isotropic thermal model: Isotropic / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Xplor3.8
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Displacement parameters | Biso mean: 44.4 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||
Refine LS restraints | *PLUS
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