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Yorodumi- PDB-1fvs: SOLUTION STRUCTURE OF THE YEAST COPPER TRANSPORTER DOMAIN CCC2A I... -
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-Basic information
Entry | Database: PDB / ID: 1fvs | ||||||
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Title | SOLUTION STRUCTURE OF THE YEAST COPPER TRANSPORTER DOMAIN CCC2A IN THE APO AND CU(I) LOAD STATES | ||||||
Components | COPPER-TRANSPORTING ATPASE | ||||||
Keywords | HYDROLASE / Cu(I)-Ccc2a / babbab | ||||||
Function / homology | Function and homology information trans-Golgi network transport vesicle membrane / Ion transport by P-type ATPases / P-type divalent copper transporter activity / P-type monovalent copper transporter activity / P-type Cu+ transporter / copper ion export / copper ion import / copper ion homeostasis / copper ion transport / ATPase-coupled monoatomic cation transmembrane transporter activity ...trans-Golgi network transport vesicle membrane / Ion transport by P-type ATPases / P-type divalent copper transporter activity / P-type monovalent copper transporter activity / P-type Cu+ transporter / copper ion export / copper ion import / copper ion homeostasis / copper ion transport / ATPase-coupled monoatomic cation transmembrane transporter activity / intracellular copper ion homeostasis / trans-Golgi network / transmembrane transport / intracellular iron ion homeostasis / copper ion binding / ATP hydrolysis activity / ATP binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing torsion angle dynamics restrained energy minimization | ||||||
Model type details | minimized average | ||||||
Authors | Banci, L. / Bertini, I. / Ciofi Baffoni, S. / Huffman, D.L. / O'Halloran, T.V. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2001 Title: Solution structure of the yeast copper transporter domain Ccc2a in the apo and Cu(I)-loaded states. Authors: Banci, L. / Bertini, I. / Ciofi-Baffoni, S. / Huffman, D.L. / O'Halloran, T.V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fvs.cif.gz | 32.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fvs.ent.gz | 21.1 KB | Display | PDB format |
PDBx/mmJSON format | 1fvs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fvs_validation.pdf.gz | 297.7 KB | Display | wwPDB validaton report |
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Full document | 1fvs_full_validation.pdf.gz | 297.4 KB | Display | |
Data in XML | 1fvs_validation.xml.gz | 2.8 KB | Display | |
Data in CIF | 1fvs_validation.cif.gz | 3.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fv/1fvs ftp://data.pdbj.org/pub/pdb/validation_reports/fv/1fvs | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7889.924 Da / Num. of mol.: 1 / Fragment: CCC2A DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Plasmid: PDLHV021 / Production host: Escherichia coli (E. coli) / References: UniProt: P38995, EC: 3.6.1.36 |
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#2: Chemical | ChemComp-CU / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.2mM Cu(I)-Ccc2a; 100mM phosphate buffer / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 100mM phosphate / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing torsion angle dynamics restrained energy minimization Software ordinal: 1 / Details: 1944 meaningful NOEs | ||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |