METAL TRANSPORT / copper protein / copper chaperone / Menkes / Wilson / Structural Proteomics in Europe / SPINE / Structural Genomics
Function / homology
Function and homology information
metallochaperone activity / Ion influx/efflux at host-pathogen interface / copper-dependent protein binding / copper chaperone activity / copper ion transport / Detoxification of Reactive Oxygen Species / cuprous ion binding / intracellular copper ion homeostasis / response to oxidative stress / copper ion binding / cytosol Similarity search - Function
Mass: 63.546 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cu
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
2
1
2
CBCANH
2
2
2
CBCA(CO)NH
2
3
2
HNCO
2
4
2
HN(CA)CO
2
5
2
(H)CCH TOCSY
2
6
2
HNHA
1
7
1
3D 15N-separated NOESY
1
8
1
2D NOESY
1
9
1
2D TOCSY
2
10
2
3D 13C-separated NOESY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1.0 mM Cu(I)HAH1 U-15N; 10 mM sodium acetate
90% H2O/10% D2O
2
2 mM Cu(I)HAH1 U-95% 13C,U-98% 15N; 4 mM DTT; 100 mM phosphate buffer
90% H2O/10% D2O
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
10mMsodiumacetate
6
ambient
298K
2
100mMphosphatebuffer
7
ambient
298K
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker AVANCE
Bruker
AVANCE
500
1
Bruker AVANCE
Bruker
AVANCE
900
2
Bruker AVANCE
Bruker
AVANCE
600
3
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Processing
NMR software
Name
Version
Classification
XwinNMR
collection
XEASY
1.3
dataanalysis
DIANA
1.5
structuresolution
CYANA
1
structuresolution
Amber
5
refinement
Refinement
Method: torsion angle dynamics, simulated annealing, restrained energy minimization Software ordinal: 1 Details: the structures were based on a total of 1219 meaningful distance constraints, 99 dihedral angle restraints.
NMR ensemble
Conformer selection criteria: target function / Conformers calculated total number: 400 / Conformers submitted total number: 30
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