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Yorodumi- PDB-2kzg: A Transient and Low Populated Protein Folding Intermediate at Ato... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kzg | ||||||
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Title | A Transient and Low Populated Protein Folding Intermediate at Atomic Resolution | ||||||
Components | Pre-mRNA-processing factor 40 homolog A | ||||||
Keywords | PROTEIN BINDING / folding intermediate / low populated / relaxation dispersion / cpmg | ||||||
Function / homology | Function and homology information mRNA cis splicing, via spliceosome / U1 snRNP / U2-type prespliceosome / cytoskeleton organization / mRNA Splicing - Major Pathway / regulation of cytokinesis / mRNA splicing, via spliceosome / nuclear matrix / cell migration / regulation of cell shape ...mRNA cis splicing, via spliceosome / U1 snRNP / U2-type prespliceosome / cytoskeleton organization / mRNA Splicing - Major Pathway / regulation of cytokinesis / mRNA splicing, via spliceosome / nuclear matrix / cell migration / regulation of cell shape / nuclear speck / cell cycle / cell division / RNA binding / nucleoplasm / membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / Monte Carlo | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Korzhnev, D.M. / Religa, T.L. / Banachewicz, W. / Fersht, A.R. / Kay, L.E. | ||||||
Citation | Journal: Science / Year: 2010 Title: A transient and low-populated protein-folding intermediate at atomic resolution. Authors: Korzhnev, D.M. / Religa, T.L. / Banachewicz, W. / Fersht, A.R. / Kay, L.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kzg.cif.gz | 194.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kzg.ent.gz | 164.4 KB | Display | PDB format |
PDBx/mmJSON format | 2kzg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kz/2kzg ftp://data.pdbj.org/pub/pdb/validation_reports/kz/2kzg | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8251.467 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Description: N-His6-Lyp-Thr-FF-C, His6 - HisTag, Lyp - lipoyl domain solublity tag, Thr - thrombin cleavage site, FF - FF domain Gene: HYPA, FBP11, PRPF40A, FLAF1, FNBP3, HIP10 / Production host: Escherichia coli (E. coli) / Strain (production host): C41 / References: UniProt: O75400 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: Atoms HG2-CG-HG3 in PRO 47 A model 6 have unusual geometry due to a bug in CS-Rosetta software. |
-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 145 / pH: 5.7 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Monte Carlo / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 10000 / Conformers submitted total number: 10 |