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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1fpr | ||||||
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タイトル | CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN THE CATALYTIC DOMAIN OF SHP-1 AND AN IN VITRO PEPTIDE SUBSTRATE PY469 DERIVED FROM SHPS-1. | ||||||
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![]() | SIGNALING PROTEIN / protein tyrosine phosphatase / substrate specificity / residue shift | ||||||
機能・相同性 | ![]() negative regulation of humoral immune response mediated by circulating immunoglobulin / negative regulation of mast cell activation involved in immune response / regulation of B cell differentiation / negative regulation of peptidyl-tyrosine phosphorylation / epididymis development / negative regulation of inflammatory response to wounding / phosphorylation-dependent protein binding / transmembrane receptor protein tyrosine phosphatase activity / natural killer cell mediated cytotoxicity / alpha-beta T cell receptor complex ...negative regulation of humoral immune response mediated by circulating immunoglobulin / negative regulation of mast cell activation involved in immune response / regulation of B cell differentiation / negative regulation of peptidyl-tyrosine phosphorylation / epididymis development / negative regulation of inflammatory response to wounding / phosphorylation-dependent protein binding / transmembrane receptor protein tyrosine phosphatase activity / natural killer cell mediated cytotoxicity / alpha-beta T cell receptor complex / regulation of release of sequestered calcium ion into cytosol / CD22 mediated BCR regulation / Interleukin-37 signaling / positive regulation of cell adhesion mediated by integrin / Costimulation by the CD28 family / Signal regulatory protein family interactions / platelet formation / negative regulation of T cell receptor signaling pathway / megakaryocyte development / negative regulation of MAPK cascade / Regulation of KIT signaling / Signaling by ALK / Platelet sensitization by LDL / regulation of G1/S transition of mitotic cell cycle / PECAM1 interactions / regulation of type I interferon-mediated signaling pathway / negative regulation of interleukin-6 production / negative regulation of tumor necrosis factor production / non-membrane spanning protein tyrosine phosphatase activity / peptidyl-tyrosine dephosphorylation / Interleukin-3, Interleukin-5 and GM-CSF signaling / Regulation of IFNA/IFNB signaling / PD-1 signaling / Interleukin receptor SHC signaling / hematopoietic progenitor cell differentiation / Nuclear events stimulated by ALK signaling in cancer / Regulation of IFNG signaling / T cell proliferation / negative regulation of T cell proliferation / Growth hormone receptor signaling / cell adhesion molecule binding / GPVI-mediated activation cascade / T cell costimulation / phosphotyrosine residue binding / SH2 domain binding / protein dephosphorylation / protein-tyrosine-phosphatase / regulation of ERK1 and ERK2 cascade / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / protein tyrosine phosphatase activity / B cell receptor signaling pathway / cytokine-mediated signaling pathway / platelet aggregation / SH3 domain binding / peptidyl-tyrosine phosphorylation / specific granule lumen / Interferon gamma signaling / MAPK cascade / Interferon alpha/beta signaling / cell-cell junction / tertiary granule lumen / mitotic cell cycle / T cell receptor signaling pathway / regulation of apoptotic process / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cell differentiation / intracellular signal transduction / G protein-coupled receptor signaling pathway / negative regulation of cell population proliferation / positive regulation of cell population proliferation / Neutrophil degranulation / nucleolus / protein kinase binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / protein-containing complex / extracellular exosome / extracellular region / nucleoplasm / membrane / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() | ||||||
![]() | Yang, J. / Cheng, Z. / Niu, Z. / Zhao, Z.J. / Zhou, G.W. | ||||||
![]() | ![]() タイトル: Structural basis for substrate specificity of protein-tyrosine phosphatase SHP-1. 著者: Yang, J. / Cheng, Z. / Niu, T. / Liang, X. / Zhao, Z.J. / Zhou, G.W. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 66.8 KB | 表示 | ![]() |
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PDB形式 | ![]() | 52.7 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 376.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 415.5 KB | 表示 | |
XML形式データ | ![]() | 12.9 KB | 表示 | |
CIF形式データ | ![]() | 18.1 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 32558.781 Da / 分子数: 1 / 断片: CATALYTIC DOMAIN / 変異: C455S / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: タンパク質・ペプチド | 分子量: 1235.146 Da / 分子数: 1 / 由来タイプ: 合成 詳細: This peptide was chemically synthesized. The sequence of this peptide occurs naturally in humans (Homo sapiens) |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.1 Å3/Da / 溶媒含有率: 41.4 % | ||||||||||||||||||||
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結晶化 | 温度: 277 K / 手法: 蒸気拡散法 / pH: 8.5 詳細: ammonium sulfate, pH 8.5, VAPOR DIFFUSION, temperature 277K | ||||||||||||||||||||
結晶化 | *PLUS | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 85 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1998年8月8日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 最高解像度: 2.5 Å / % possible obs: 85 % / Rmerge(I) obs: 0.097 |
反射 | *PLUS Num. obs: 8998 / % possible obs: 85 % / Num. measured all: 27345 |
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解析
ソフトウェア |
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精密化 | 解像度: 2.5→6 Å / σ(F): 2
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精密化ステップ | サイクル: LAST / 解像度: 2.5→6 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.5 Å / 最低解像度: 6 Å / σ(F): 2 / Rfactor all: 0.199 / Rfactor obs: 0.194 | ||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||
拘束条件 | *PLUS タイプ: x_angle_deg / Dev ideal: 1.4 |