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- PDB-1f9u: CRYSTAL STRUCTURES OF MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f9u | ||||||
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Title | CRYSTAL STRUCTURES OF MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACTIVATION OF THE KINESIN MOTOR ATPASE | ||||||
![]() | KINESIN-LIKE PROTEIN KAR3 | ||||||
![]() | CONTRACTILE PROTEIN / Kar3 / Kinesin-related protein / motor protein / microtubule binding protein | ||||||
Function / homology | ![]() minus-end-directed kinesin ATPase / microtubule bundle formation involved in mitotic spindle midzone assembly / nuclear migration involved in conjugation with cellular fusion / protein transport along microtubule to mitotic spindle pole body / karyogamy involved in conjugation with cellular fusion / minus-end-directed microtubule motor activity / spindle pole body / mitotic sister chromatid cohesion / cytoplasmic microtubule / regulation of mitotic spindle organization ...minus-end-directed kinesin ATPase / microtubule bundle formation involved in mitotic spindle midzone assembly / nuclear migration involved in conjugation with cellular fusion / protein transport along microtubule to mitotic spindle pole body / karyogamy involved in conjugation with cellular fusion / minus-end-directed microtubule motor activity / spindle pole body / mitotic sister chromatid cohesion / cytoplasmic microtubule / regulation of mitotic spindle organization / isomerase activity / meiotic cell cycle / spindle pole / chromosome / mitotic cell cycle / microtubule binding / microtubule / cell division / ATP hydrolysis activity / ATP binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Yun, M. / Zhang, X. / Park, C.G. / Park, H.W. / Endow, S.A. | ||||||
![]() | ![]() Title: A structural pathway for activation of the kinesin motor ATPase. Authors: Yun, M. / Zhang, X. / Park, C.G. / Park, H.W. / Endow, S.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 81 KB | Display | ![]() |
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PDB format | ![]() | 58.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 765 KB | Display | ![]() |
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Full document | ![]() | 774.6 KB | Display | |
Data in XML | ![]() | 16.6 KB | Display | |
Data in CIF | ![]() | 23.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38978.809 Da / Num. of mol.: 1 / Fragment: N650K MUTANT MOTOR DOMAIN / Mutation: L383M, N650K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: SACCHAROMYCES CEREVISIAE / Plasmid: PMW174 / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-ADP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.21 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: HEPES, PEG 2000 ME, Ethylen Glycol, magnesium chloride, Sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / PH range low: 8 / PH range high: 7 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: CCD / Date: Apr 21, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→20 Å / Num. all: 31822 / Num. obs: 31822 / % possible obs: 93.5 % / Observed criterion σ(I): -3 / Redundancy: 9.72 % / Rmerge(I) obs: 0.069 / Net I/σ(I): 17.5 |
Reflection shell | Resolution: 1.7→1.73 Å / Redundancy: 2.95 % / Num. unique all: 1519 / % possible all: 89.5 |
Reflection | *PLUS Num. measured all: 309408 |
Reflection shell | *PLUS % possible obs: 89.5 % |
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Processing
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Refinement | Resolution: 1.7→20 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.7→20 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 20 Å / σ(F): 2 / % reflection Rfree: 10 % / Rfactor obs: 0.217 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |