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Yorodumi- PDB-1f9t: CRYSTAL STRUCTURES OF KINESIN MUTANTS REVEAL A SIGNALLING PATHWAY... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f9t | ||||||
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Title | CRYSTAL STRUCTURES OF KINESIN MUTANTS REVEAL A SIGNALLING PATHWAY FOR ACTIVATION OF THE MOTOR ATPASE | ||||||
Components | KINESIN-LIKE PROTEIN KAR3 | ||||||
Keywords | CONTRACTILE PROTEIN / Kar3 / Kinesin-related protein / motor protein / microtubule binding protein | ||||||
Function / homology | Function and homology information minus-end-directed kinesin ATPase / microtubule bundle formation involved in mitotic spindle midzone assembly / nuclear migration involved in conjugation with cellular fusion / protein transport along microtubule to mitotic spindle pole body / karyogamy involved in conjugation with cellular fusion / minus-end-directed microtubule motor activity / spindle pole body / mitotic sister chromatid cohesion / cytoplasmic microtubule / regulation of mitotic spindle organization ...minus-end-directed kinesin ATPase / microtubule bundle formation involved in mitotic spindle midzone assembly / nuclear migration involved in conjugation with cellular fusion / protein transport along microtubule to mitotic spindle pole body / karyogamy involved in conjugation with cellular fusion / minus-end-directed microtubule motor activity / spindle pole body / mitotic sister chromatid cohesion / cytoplasmic microtubule / regulation of mitotic spindle organization / isomerase activity / meiotic cell cycle / spindle pole / mitotic cell cycle / chromosome / microtubule binding / microtubule / cell division / ATP hydrolysis activity / ATP binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.5 Å | ||||||
Authors | Yun, M. / Zhang, X. / Park, C.-G. / Park, H.-W. / Endow, S.A. | ||||||
Citation | Journal: EMBO J. / Year: 2001 Title: A structural pathway for activation of the kinesin motor ATPase. Authors: Yun, M. / Zhang, X. / Park, C.G. / Park, H.W. / Endow, S.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f9t.cif.gz | 82.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f9t.ent.gz | 59.2 KB | Display | PDB format |
PDBx/mmJSON format | 1f9t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f9/1f9t ftp://data.pdbj.org/pub/pdb/validation_reports/f9/1f9t | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 40357.293 Da / Num. of mol.: 1 / Fragment: MOTOR DOMAIN / Mutation: V372M Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Plasmid: PMW174 / Production host: Escherichia coli (E. coli) / References: UniProt: P17119 |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-ADP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.59 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: HEPES, PEG 2000 ME, magnesium chloride, Ethylen glycol, Sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / PH range low: 8 / PH range high: 7 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.9795 |
Detector | Detector: CCD / Date: Jan 1, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→20 Å / Num. all: 47889 / Num. obs: 47889 / % possible obs: 97.3 % / Observed criterion σ(I): -3 / Redundancy: 16.6 % / Rmerge(I) obs: 0.074 / Net I/σ(I): 16.2 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 2 % / Num. unique all: 2343 / % possible all: 94.9 |
Reflection | *PLUS Num. measured all: 793427 |
Reflection shell | *PLUS % possible obs: 94.9 % |
-Processing
Software |
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Refinement | Resolution: 1.5→20 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.5→20 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.5 Å / Lowest resolution: 20 Å / σ(F): 2 / % reflection Rfree: 10 % / Rfactor obs: 0.213 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |