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Yorodumi- PDB-1f6f: CRYSTAL STRUCTURE OF THE TERNARY COMPLEX BETWEEN OVINE PLACENTAL ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f6f | ||||||
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Title | CRYSTAL STRUCTURE OF THE TERNARY COMPLEX BETWEEN OVINE PLACENTAL LACTOGEN AND THE EXTRACELLULAR DOMAIN OF THE RAT PROLACTIN RECEPTOR | ||||||
Components |
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Keywords | HORMONE/GROWTH FACTOR/HORMONE RECEPTOR / 4-helical bundle / alpha helical bundle / ternary complex / FN III domains / beta sheet domains / cytokine-receptor complex / HORMONE-GROWTH FACTOR-HORMONE RECEPTOR COMPLEX | ||||||
Function / homology | Function and homology information Prolactin receptor signaling / prolactin receptor activity / prolactin receptor binding / Growth hormone receptor signaling / regulation of epithelial cell differentiation / mammary gland epithelium development / prostate gland growth / mammary gland epithelial cell differentiation / cellular response to granulocyte macrophage colony-stimulating factor stimulus / cytokine binding ...Prolactin receptor signaling / prolactin receptor activity / prolactin receptor binding / Growth hormone receptor signaling / regulation of epithelial cell differentiation / mammary gland epithelium development / prostate gland growth / mammary gland epithelial cell differentiation / cellular response to granulocyte macrophage colony-stimulating factor stimulus / cytokine binding / peptide hormone binding / cell surface receptor signaling pathway via JAK-STAT / mammary gland alveolus development / regulation of cell adhesion / positive regulation of B cell proliferation / positive regulation of protein autophosphorylation / lactation / response to bacterium / hormone activity / cytokine-mediated signaling pathway / positive regulation of cold-induced thermogenesis / receptor complex / external side of plasma membrane / lipid binding / positive regulation of cell population proliferation / negative regulation of apoptotic process / protein kinase binding / cell surface / extracellular region / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Ovis aries (sheep) Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.3 Å | ||||||
Authors | Elkins, P.A. / Christinger, H.W. / Sandowski, Y. / Sakal, E. / Gertler, A. / De Vos, A.M. / Kossiakoff, A.A. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Ternary complex between placental lactogen and the extracellular domain of the prolactin receptor. Authors: Elkins, P.A. / Christinger, H.W. / Sandowski, Y. / Sakal, E. / Gertler, A. / de Vos, A.M. / Kossiakoff, A.A. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1998 Title: Crystallization of Ovine Placental Lactogen in a 1:2 Complex with the Extracellular Domain of the Rat Prolactin Receptor Authors: Christinger, H.W. / Elkins, P.A. / Sandowski, Y. / Sakal, E. / Gertler, A. / Kossiakoff, A.A. / De Vos, A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f6f.cif.gz | 120 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f6f.ent.gz | 100.6 KB | Display | PDB format |
PDBx/mmJSON format | 1f6f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1f6f_validation.pdf.gz | 380.9 KB | Display | wwPDB validaton report |
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Full document | 1f6f_full_validation.pdf.gz | 395.3 KB | Display | |
Data in XML | 1f6f_validation.xml.gz | 13.1 KB | Display | |
Data in CIF | 1f6f_validation.cif.gz | 20.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f6/1f6f ftp://data.pdbj.org/pub/pdb/validation_reports/f6/1f6f | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a complex of the hormone (chain A) bound to 2 copies of the receptor (chains B and C). The copies of the receptor are not related by precise symmetry. |
-Components
#1: Protein | Mass: 22601.643 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ovis aries (sheep) / Plasmid: PET8 / Production host: Escherichia coli (E. coli) / References: UniProt: P16038 | ||
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#2: Protein | Mass: 24584.494 Da / Num. of mol.: 2 Fragment: EXTRACELLULAR DOMAIN; N-TERMINAL FIBRONECTIN TYPE III DOMAINS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Organ: LIVER / Plasmid: PTRC99A / Production host: Escherichia coli (E. coli) / References: UniProt: P05710 #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.87 Å3/Da / Density % sol: 57.1 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: PEG 4000, isopropanol, MPD, MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K | |||||||||||||||||||||||||
Crystal grow | *PLUS Details: Christinger, H.W., (1998) Acta Crystallogr., Sect.D, 54, 1408. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: A1 |
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Detector | Type: ADSC / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.3→30 Å / Num. all: 36413 / Num. obs: 33034 / % possible obs: 90.7 % / Biso Wilson estimate: 47.9 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 16.2 |
Reflection shell | Resolution: 2.3→30 Å / Rmerge(I) obs: 0.13 / Num. unique all: 2537 / % possible all: 70.4 |
Reflection shell | *PLUS % possible obs: 70.4 % |
-Processing
Software |
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Refinement | Resolution: 2.3→30 Å / σ(F): 0.01 / Stereochemistry target values: Engh & Huber Details: Model bias was reduced by adding experimental phase information using cross-crystal averaging from a non-isomorphous mercury derivative.
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Refinement step | Cycle: LAST / Resolution: 2.3→30 Å
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Refine LS restraints |
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