Mass: 24490.309 Da / Num. of mol.: 1 / Fragment: DBL HOMOLOGY DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: PET11A / Production host: Escherichia coli (E. coli) / References: UniProt: P27870
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
4D 13C-separated NOESY
1
2
1
4D 13C/15N-separated NOESY
1
3
1
3D 15N-separated NOESY
1
4
1
3D 13C-separated NOESY
1
5
1
2D NOESY
NMR details
Text: This structure was determined using 3D and 4D heteronuclear techniques on deuterated samples in conjunction with selective methyl and aromatic labeling
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Sample preparation
Details
Contents: 1.4 mM 15N,13C,2H; 20 mM phosphate buffer; 50 mM NaCl; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O
Sample conditions
Ionic strength: 70 mM / pH: 7 / Pressure: ambient / Temperature: 298 K
Method: torsion angle dynamics / Software ordinal: 1 Details: CNS was used in the initial global fold determination. High-resolution structures were obtained using ARIA. Final structures are based on a total of 3966 restraints, 3523 are NOE-derived ...Details: CNS was used in the initial global fold determination. High-resolution structures were obtained using ARIA. Final structures are based on a total of 3966 restraints, 3523 are NOE-derived distance constraints, 443 dihedral angle restraints, 96 distance restraints
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20
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