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- PDB-1f2k: CRYSTAL STRUCTURE OF ACANTHAMOEBA CASTELLANII PROFILIN II, CUBIC ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f2k | ||||||
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Title | CRYSTAL STRUCTURE OF ACANTHAMOEBA CASTELLANII PROFILIN II, CUBIC CRYSTAL FORM | ||||||
![]() | PROFILIN II | ||||||
![]() | STRUCTURAL PROTEIN / SEVEN-STRANDED INCOMPLETE ANTIPARALLEL UP-AND-DOWN BETA BARREL / ACTIN-BINDING PROTEIN / POLY-L-PROLINE BINDING PROTEIN / PIP2 BINDING PROTEIN | ||||||
Function / homology | ![]() sequestering of actin monomers / actin monomer binding / cell cortex / cytoskeleton Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fedorov, A.A. / Shi, W. / Mahoney, N. / Kaiser, D.A. / Almo, S.C. | ||||||
![]() | ![]() Title: A Comparative Structural Analysis of Profilins Authors: Fedorov, A.A. / Shi, W. / Mahoney, N. / Kaiser, D.A. / Almo, S.C. #1: ![]() Title: X-ray Structures of Isoforms of the Actin-Binding Protein Profilin that Differ in their Affinity for Phosphatidylinositol Phosphates Authors: Fedorov, A.A. / Magnus, K.A. / Graupe, M.H. / Lattman, E.E. / Pollard, T.D. / Almo, S.C. #2: ![]() Title: Purification, Characterization and Crystallization of Human Platelet Profilin Expressed in Escherichia coli Authors: Fedorov, A.A. / Pollard, T.D. / Almo, S.C. #3: ![]() Title: The Molecular Basis for Allergen Cross-Reactivity: Crystal Structure and IgE-Epitope Mapping of Birch Pollen Profilin Authors: Fedorov, A.A. / Ball, T. / Mahoney, N. / Valenta, R. / Almo, S.C. #4: ![]() Title: Crystal Packing Induces a Conformational Change in Profilin-I from Acanthamoeba Castellanii Authors: Liu, S. / Fedorov, A.A. / Pollard, T.D. / Lattman, E.E. / Almo, S.C. / Magnus, K.A. #5: ![]() Title: Profilin Binds Proline-Rich Ligands in Two Distinct Amido Backbone Orientations Authors: Mahoney, N.M. / Rozwarski, D.A. / Fedorov, E.V. / Fedorov, A.A. / Almo, S.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 62.3 KB | Display | ![]() |
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PDB format | ![]() | 45.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 374.4 KB | Display | ![]() |
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Full document | ![]() | 380.2 KB | Display | |
Data in XML | ![]() | 6.6 KB | Display | |
Data in CIF | ![]() | 10.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1k0kC ![]() 2acgS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is a monomer constructed from chain A or from chain B |
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Components
#1: Protein | Mass: 12937.442 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 54 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: AMMONIUM SULFATE, SODIUM CITRATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
-Data collection
Diffraction | Mean temperature: 140 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Feb 21, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. all: 13681 / Num. obs: 13681 / % possible obs: 99 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 19.2 Å2 / Rmerge(I) obs: 0.073 / Net I/σ(I): 18.3 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.195 / Mean I/σ(I) obs: 7.2 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2ACG Resolution: 2.3→20 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||
Xplor file | Serial no: 1 / Param file: PROTEIN_REP.PARAM / Topol file: PROTEIN.TOP |