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Yorodumi- PDB-1f2k: CRYSTAL STRUCTURE OF ACANTHAMOEBA CASTELLANII PROFILIN II, CUBIC ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f2k | ||||||
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Title | CRYSTAL STRUCTURE OF ACANTHAMOEBA CASTELLANII PROFILIN II, CUBIC CRYSTAL FORM | ||||||
Components | PROFILIN II | ||||||
Keywords | STRUCTURAL PROTEIN / SEVEN-STRANDED INCOMPLETE ANTIPARALLEL UP-AND-DOWN BETA BARREL / ACTIN-BINDING PROTEIN / POLY-L-PROLINE BINDING PROTEIN / PIP2 BINDING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Acanthamoeba castellanii (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Fedorov, A.A. / Shi, W. / Mahoney, N. / Kaiser, D.A. / Almo, S.C. | ||||||
Citation | Journal: To be Published Title: A Comparative Structural Analysis of Profilins Authors: Fedorov, A.A. / Shi, W. / Mahoney, N. / Kaiser, D.A. / Almo, S.C. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 Title: X-ray Structures of Isoforms of the Actin-Binding Protein Profilin that Differ in their Affinity for Phosphatidylinositol Phosphates Authors: Fedorov, A.A. / Magnus, K.A. / Graupe, M.H. / Lattman, E.E. / Pollard, T.D. / Almo, S.C. #2: Journal: J.Mol.Biol. / Year: 1994 Title: Purification, Characterization and Crystallization of Human Platelet Profilin Expressed in Escherichia coli Authors: Fedorov, A.A. / Pollard, T.D. / Almo, S.C. #3: Journal: Structure / Year: 1997 Title: The Molecular Basis for Allergen Cross-Reactivity: Crystal Structure and IgE-Epitope Mapping of Birch Pollen Profilin Authors: Fedorov, A.A. / Ball, T. / Mahoney, N. / Valenta, R. / Almo, S.C. #4: Journal: J.STRUCT.BIOL. / Year: 1998 Title: Crystal Packing Induces a Conformational Change in Profilin-I from Acanthamoeba Castellanii Authors: Liu, S. / Fedorov, A.A. / Pollard, T.D. / Lattman, E.E. / Almo, S.C. / Magnus, K.A. #5: Journal: NAT.STRUCT.BIOL. / Year: 1999 Title: Profilin Binds Proline-Rich Ligands in Two Distinct Amido Backbone Orientations Authors: Mahoney, N.M. / Rozwarski, D.A. / Fedorov, E.V. / Fedorov, A.A. / Almo, S.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f2k.cif.gz | 62.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f2k.ent.gz | 45.9 KB | Display | PDB format |
PDBx/mmJSON format | 1f2k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f2/1f2k ftp://data.pdbj.org/pub/pdb/validation_reports/f2/1f2k | HTTPS FTP |
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-Related structure data
Related structure data | 1k0kC 2acgS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | The biological assembly is a monomer constructed from chain A or from chain B |
-Components
#1: Protein | Mass: 12937.442 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acanthamoeba castellanii (eukaryote) / Production host: Escherichia coli (E. coli) / References: UniProt: P19984 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 54 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: AMMONIUM SULFATE, SODIUM CITRATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
-Data collection
Diffraction | Mean temperature: 140 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X9B / Wavelength: 1.2 |
Detector | Type: FUJI / Detector: IMAGE PLATE / Date: Feb 21, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. all: 13681 / Num. obs: 13681 / % possible obs: 99 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 19.2 Å2 / Rmerge(I) obs: 0.073 / Net I/σ(I): 18.3 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.195 / Mean I/σ(I) obs: 7.2 / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2ACG Resolution: 2.3→20 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||
Xplor file | Serial no: 1 / Param file: PROTEIN_REP.PARAM / Topol file: PROTEIN.TOP |