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Open data
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Basic information
Entry | Database: PDB / ID: 2acg | ||||||
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Title | ACANTHAMOEBA CASTELLANII PROFILIN II | ||||||
![]() | PROFILIN II | ||||||
![]() | CONTRACTILE PROTEIN / PROTEIN BINDING / PROFILIN / ACTIN-BINDING PROTEIN | ||||||
Function / homology | ![]() sequestering of actin monomers / actin monomer binding / cell cortex / cytoskeleton Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Fedorov, A.A. / Magnus, K.A. / Graupe, M.H. / Lattman, E.E. / Pollard, T.D. / Almo, S.C. | ||||||
![]() | ![]() Title: X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates. Authors: Fedorov, A.A. / Magnus, K.A. / Graupe, M.H. / Lattman, E.E. / Pollard, T.D. / Almo, S.C. #1: ![]() Title: Analysis of Cdna Clones for Acanthamoeba Profilin-I and Profilin-II Shows End to End Homology with Vertebrate Profilins and a Small Family of Profilin Genes Authors: Pollard, T.D. / Rimm, D.L. #2: ![]() Title: The Primary Structure of the Basic Isoform of Acanthamoeba Profilin Authors: Ampe, C. / Sato, M. / Pollard, T.D. / Vanderkerckhove, J. | ||||||
History |
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Remark 700 | SHEET THE SEVEN-STRANDED SHEET S1 DESCRIBED BELOW IS A SEVEN-STRANDED INCOMPLETE ANTIPARALLEL UP- ...SHEET THE SEVEN-STRANDED SHEET S1 DESCRIBED BELOW IS A SEVEN-STRANDED INCOMPLETE ANTIPARALLEL UP-AND-DOWN BETA BARREL. TWO STRANDS HAVE BETA-BULGES: LEU 70, ARG 71 AND TRP 29, ALA 30. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 34.4 KB | Display | ![]() |
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PDB format | ![]() | 23.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 360.7 KB | Display | ![]() |
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Full document | ![]() | 364.4 KB | Display | |
Data in XML | ![]() | 4.3 KB | Display | |
Data in CIF | ![]() | 6.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12937.442 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Water | ChemComp-HOH / |
Sequence details | THE SEQUENCE WAS DERIVED FROM THAT OF POLLARD AND RIMM, CELL MOTILITY AND THE CYTOSKELETON, VOL. ...THE SEQUENCE WAS DERIVED FROM THAT OF POLLARD AND RIMM, CELL MOTILITY AND THE CYTOSKELET |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.54 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Num. obs: 4479 / Observed criterion σ(I): 2 |
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Processing
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Refinement | Resolution: 2.5→10 Å / σ(F): 2 /
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Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 18.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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