+Open data
-Basic information
Entry | Database: PDB / ID: 2acg | ||||||
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Title | ACANTHAMOEBA CASTELLANII PROFILIN II | ||||||
Components | PROFILIN II | ||||||
Keywords | CONTRACTILE PROTEIN / PROTEIN BINDING / PROFILIN / ACTIN-BINDING PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Acanthamoeba castellanii (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | ||||||
Authors | Fedorov, A.A. / Magnus, K.A. / Graupe, M.H. / Lattman, E.E. / Pollard, T.D. / Almo, S.C. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 Title: X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates. Authors: Fedorov, A.A. / Magnus, K.A. / Graupe, M.H. / Lattman, E.E. / Pollard, T.D. / Almo, S.C. #1: Journal: Cell Motil.Cytoskeleton / Year: 1991 Title: Analysis of Cdna Clones for Acanthamoeba Profilin-I and Profilin-II Shows End to End Homology with Vertebrate Profilins and a Small Family of Profilin Genes Authors: Pollard, T.D. / Rimm, D.L. #2: Journal: Eur.J.Biochem. / Year: 1988 Title: The Primary Structure of the Basic Isoform of Acanthamoeba Profilin Authors: Ampe, C. / Sato, M. / Pollard, T.D. / Vanderkerckhove, J. | ||||||
History |
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Remark 700 | SHEET THE SEVEN-STRANDED SHEET S1 DESCRIBED BELOW IS A SEVEN-STRANDED INCOMPLETE ANTIPARALLEL UP- ...SHEET THE SEVEN-STRANDED SHEET S1 DESCRIBED BELOW IS A SEVEN-STRANDED INCOMPLETE ANTIPARALLEL UP-AND-DOWN BETA BARREL. TWO STRANDS HAVE BETA-BULGES: LEU 70, ARG 71 AND TRP 29, ALA 30. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2acg.cif.gz | 34.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2acg.ent.gz | 23.8 KB | Display | PDB format |
PDBx/mmJSON format | 2acg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ac/2acg ftp://data.pdbj.org/pub/pdb/validation_reports/ac/2acg | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12937.442 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acanthamoeba castellanii (eukaryote) / Gene: CDNA / References: UniProt: P19984 |
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#2: Water | ChemComp-HOH / |
Sequence details | THE SEQUENCE WAS DERIVED FROM THAT OF POLLARD AND RIMM, CELL MOTILITY AND THE CYTOSKELETON, VOL. ...THE SEQUENCE WAS DERIVED FROM THAT OF POLLARD AND RIMM, CELL MOTILITY AND THE CYTOSKELET |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.54 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Num. obs: 4479 / Observed criterion σ(I): 2 |
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-Processing
Software |
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Refinement | Resolution: 2.5→10 Å / σ(F): 2 /
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Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 18.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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