Journal: Ph D Thesis / Year: 1999 Title: Refinement of Four Endothiapepsin Inhibitor Complexes. Crystallographic Studies of Cytochrome Ch from Methylobacterium Extorquens and Inhibitor Complexes of Aspartic Proteinases. Authors: Read, J.A.
History
Deposition
Sep 15, 2000
Deposition site: PDBE / Processing site: PDBE
Revision 1.0
Sep 26, 2000
Provider: repository / Type: Initial release
Revision 1.1
Jul 13, 2011
Group: Atomic model / Database references ...Atomic model / Database references / Derived calculations / Non-polymer description / Structure summary / Version format compliance
Mass: 18.015 Da / Num. of mol.: 166 / Source method: isolated from a natural source / Formula: H2O
Compound details
CATALYTIC ACTIVITY: HYDROLYSIS OF PROTEINS WITH BROAD SPECIFICITY SIMILAR TO THAT OF PEPSIN A, ...CATALYTIC ACTIVITY: HYDROLYSIS OF PROTEINS WITH BROAD SPECIFICITY SIMILAR TO THAT OF PEPSIN A, PREFERRING HYDROPHOBIC RESIDUES AT AND P1', BUT DOES NOT CLEAVE 14-ALA-|-LEU-15 IN THE B CHAIN OF INSULIN OR Z-GLU-TYR. CLOTS MILK. SIMILARITY: BELONGS TO PEPTIDASE FAMILY A1; ALSO KNOWN AS THE EUKARYOTIC ASPARTYL PROTEASES FAMILY.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.55 Å3/Da / Density % sol: 51.7 %
Crystal grow
pH: 5 Details: BATCH METHOD PROTEIN: 10MG/ML, 50 MM AMMONIUM ACETATE PH 5.0, ~2.2 M AMMONIUM SULPHATE, 1% ACETONE. INHIBITOR WAS ADDED IN A 10:1 STOICHIOMETRIC RATIO.
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Data collection
Diffraction
Mean temperature: 298 K
Diffraction source
Source: ROTATING ANODE / Wavelength: 1.5418
Detector
Type: MARRESEARCH / Detector: IMAGE PLATE
Radiation
Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.5418 Å / Relative weight: 1
Reflection
Resolution: 2.05→30 Å / Num. obs: 21735 / % possible obs: 93.1 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Rsym value: 0.102 / Net I/σ(I): 6.5
Reflection shell
Resolution: 2.05→2.29 Å / Redundancy: 4 % / Mean I/σ(I) obs: 2.2 / Rsym value: 0.329 / % possible all: 90.5
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