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- PDB-1e7k: Crystal structure of the spliceosomal 15.5kD protein bound to a U... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1e7k | ||||||
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Title | Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment | ||||||
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![]() | RNA BINDING PROTEIN / RNA-BINDING PROTEIN / SPLICING / RNA RECOGNITION MOTIF / U4 SNRNA | ||||||
Function / homology | ![]() U4atac snRNP / U4atac snRNA binding / box C/D sno(s)RNA binding / dense fibrillar component / U4 snRNA binding / box C/D methylation guide snoRNP complex / U2-type precatalytic spliceosome / box C/D snoRNP assembly / rRNA modification in the nucleus and cytosol / U3 snoRNA binding ...U4atac snRNP / U4atac snRNA binding / box C/D sno(s)RNA binding / dense fibrillar component / U4 snRNA binding / box C/D methylation guide snoRNP complex / U2-type precatalytic spliceosome / box C/D snoRNP assembly / rRNA modification in the nucleus and cytosol / U3 snoRNA binding / precatalytic spliceosome / single fertilization / Major pathway of rRNA processing in the nucleolus and cytosol / U4/U6 x U5 tri-snRNP complex / mRNA Splicing - Major Pathway / maturation of LSU-rRNA / maturation of SSU-rRNA / small-subunit processome / mRNA splicing, via spliceosome / ribosomal small subunit biogenesis / ATPase binding / cytosolic large ribosomal subunit / nucleolus / protein-containing complex / RNA binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vidovic, I. / Nottrott, S. / Harthmuth, K. / Luhrmann, R. / Ficner, R. | ||||||
![]() | ![]() Title: Crystal Structure of the Spliceosomal 15.5Kd Protein Bound to a U4 Snrna Fragment Authors: Vidovic, I. / Nottrott, S. / Hartmuth, K. / Luhrmann, R. / Ficner, R. #1: Journal: Embo J. / Year: 1999 Title: Functional Interaction of a Novel 15.5Kd [U4/U6.U5] Tri-Snrnp Protein with the 5' Stem-Loop of U4 Snrna Authors: Nottrott, S. / Hartmuth, K. / Fabrizio, P. / Urlaub, H. / Vidovic, I. / Ficner, R. / Luhrmann, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.4 KB | Display | ![]() |
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PDB format | ![]() | 57.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 404.8 KB | Display | ![]() |
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Full document | ![]() | 417.5 KB | Display | |
Data in XML | ![]() | 8 KB | Display | |
Data in CIF | ![]() | 12.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.057157, 0.330472, -0.942084), Vector: |
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Components
#1: Protein | Mass: 14191.524 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: RNA chain | Mass: 7074.250 Da / Num. of mol.: 2 / Fragment: 26-47 / Source method: obtained synthetically / Source: (synth.) ![]() Sequence details | THE SWISSPROT ENTRY P55769 IS IDENTICAL TO THE SWISSPROT ENTRY AAF06959 REPORTED IN, S.NOTTROTT,K. ...THE SWISSPROT ENTRY P55769 IS IDENTICAL TO THE SWISSPROT ENTRY AAF06959 REPORTED IN, S.NOTTROTT,K.HARTMUTH,P.FABRIZIO,H.URLAUB,I.VIDOVIC, R.FICNER,R.LUHRMANN FUNCTIONAL | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 37 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.6 / Details: pH 7.60 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 21 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Date: Mar 15, 1999 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.9→30 Å / Num. obs: 8125 / % possible obs: 93.2 % / Redundancy: 3.5 % / Rsym value: 0.055 | |||||||||||||||
Reflection shell | Resolution: 2.9→3 Å / Rsym value: 0.118 / % possible all: 89.3 | |||||||||||||||
Reflection | *PLUS Num. measured all: 28438 / Rmerge(I) obs: 0.055 | |||||||||||||||
Reflection shell | *PLUS % possible obs: 89.3 % / Rmerge(I) obs: 0.118 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 15.192 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→30 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS % reflection Rfree: 10 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |