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- PDB-6eez: Crystal Structure of the thiol-disulfide exchange protein alpha-D... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6eez | ||||||
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Title | Crystal Structure of the thiol-disulfide exchange protein alpha-DsbA2 from Wolbachia pipientis | ||||||
![]() | DsbA-like disulfide oxidoreductase | ||||||
![]() | ISOMERASE / Thiol-disulfide exchange protein | ||||||
Function / homology | Thioredoxin / Thioredoxin-like fold / Thioredoxin-like superfamily / DsbA-like disulfide oxidoreductase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Walden, P.M. / Lakshmanane, P. / King, G.J. / Martin, J.L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The atypical thiol-disulfide exchange protein alpha-DsbA2 from Wolbachia pipientis is a homotrimeric disulfide isomerase. Authors: Walden, P.M. / Whitten, A.E. / Premkumar, L. / Halili, M.A. / Heras, B. / King, G.J. / Martin, J.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 420.8 KB | Display | ![]() |
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PDB format | ![]() | 365.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.5 KB | Display | ![]() |
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Full document | ![]() | 459.6 KB | Display | |
Data in XML | ![]() | 28.5 KB | Display | |
Data in CIF | ![]() | 40.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21122.807 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: WD_1312 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.8 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 100 mM Tris pH 7.5, 200 mM NaCl and 20% w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 15, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.543 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→19.77 Å / Num. obs: 35424 / % possible obs: 99.04 % / Redundancy: 3.8 % / Biso Wilson estimate: 58 Å2 / Rmerge(I) obs: 0.051 / Rpim(I) all: 0.038 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 2.25→2.32 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.265 / Mean I/σ(I) obs: 3.9 / Num. unique obs: 3209 / Rsym value: 0.187 / % possible all: 97.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.25→19.768 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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