+Open data
-Basic information
Entry | Database: PDB / ID: 1e5o | ||||||
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Title | Endothiapepsin complex with inhibitor DB2 | ||||||
Components | ENDOTHIAPEPSIN | ||||||
Keywords | HYDROLASE/HYDROLASE INHIBITOR / HYDROLASE / ACID PROTEINASE / HYDROLASE-HYDROLASE INHIBITOR COMPLEX | ||||||
Function / homology | Function and homology information | ||||||
Biological species | ENDOTHIA PARASITICA (chestnut blight fungus) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.05 Å | ||||||
Authors | Read, J.A. / Cooper, J.B. / Toldo, L. / Bailey, D. | ||||||
Citation | Journal: Thesis / Year: 1999 Title: Refinement of Four Endothiapepsin Inhibitor Complexes. Crystallographic Studies of Cytochrome Ch from Methylobacterium Extorquens and Inhibitor Complexes of Aspartic Proteinases. Authors: Read, J.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e5o.cif.gz | 78 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e5o.ent.gz | 56.3 KB | Display | PDB format |
PDBx/mmJSON format | 1e5o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e5/1e5o ftp://data.pdbj.org/pub/pdb/validation_reports/e5/1e5o | HTTPS FTP |
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-Related structure data
Related structure data | 1e81C 1e82C 4apeS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 33813.855 Da / Num. of mol.: 1 / Fragment: RESIDUE 90-419 / Source method: isolated from a natural source Source: (natural) ENDOTHIA PARASITICA (chestnut blight fungus) References: UniProt: P11838, mucorpepsin |
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#2: Chemical | ChemComp-3AI / |
#3: Water | ChemComp-HOH / |
Compound details | CATALYTIC ACTIVITY: HYDROLYSIS OF PROTEINS WITH BROAD SPECIFICITY SIMILAR TO THAT OF PEPSIN A, ...CATALYTIC ACTIVITY: HYDROLYSIS |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.5 % |
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Crystal grow | pH: 5 Details: BATCH METHOD PROTEIN: 10MG/ML, 50 MM AMMONIUM ACETATE PH 5.0, ~2.2 M AMMONIUM SULPHATE, 1% ACETONE. INHIBITOR WAS ADDED IN A 10:1 STOICHIOMETRIC RATIO. |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→30 Å / Num. obs: 21510 / % possible obs: 98.9 % / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Rsym value: 0.123 / Net I/σ(I): 5.3 |
Reflection shell | Resolution: 2.05→2.29 Å / Redundancy: 3.7 % / Mean I/σ(I) obs: 1.6 / Rsym value: 0.461 / % possible all: 94.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4APE Resolution: 2.05→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BOND LENGTHS (A) : .0003
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Refinement step | Cycle: LAST / Resolution: 2.05→30 Å
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